Research Article

Interleukin-27 Protects Cardiomyocyte-Like H9c2 Cells against Metabolic Syndrome: Role of STAT3 Signaling

Figure 1

Cytotoxic effects of high glucose, high sodium, or high cholesterol on the viability of H9c2 cells. (a) Cells were treated with glucose (25 mM, G), NaCl (250 mM, S), or cholesterol (300 μg mL−1, C), either alone or in different combinations, for 4, 8, 24, and 48 h. Cells were treated with IL-27 4 h before treatment with G, S, or C. Lactate dehydrogenase (LDH) release was measured as marker of cell injury. (b) Cells were cultured for 48 h in the presence of G, S, or C (either alone or in different combinations) and cell viability was monitored in an MTT assay. of experiments performed at each time point. versus the control group; versus G; versus S; versus C; versus GS; versus GC; versus the SC; and versus the vehicle-treated control at the same time-point.