Review Article
Two-Dimensional Crystallization Procedure, from Protein Expression to Sample Preparation
Figure 3
Negatively stained rMGST1 crystals. The effects of different salt concentrations and dialysis temperatures are shown in the figure. (a) Previous condition [37]. (b) With additional CaCl2. (c) With an increased dialysis temperature. (d) Combined effect of salt and higher temperature. The size of the crystals improves significantly in (d) as compared to (a–c). A number of single layer sheets were obtained in (d), making data collection possible. The crystals in these four conditions were embedded in 1% uranyl acetate. These four images were taken at the same nominal magnification of 5000x and the scale bar is 2 m.
(a) |
(b) |
(c) |
(d) |