Research Article
Preoperative RAS Mutational Analysis Is of Great Value in Predicting Follicular Variant of Papillary Thyroid Carcinoma
Table 1
PCR primers for BRAF and RAS mutational analyses.
| | Gene | Codon | | PCR primer sequences |
| | BRAF | 600/601 | F | 5′-B-CTTCATAATGCTTGCTCTGATAGG-3′ | | | R | 5′-GGCCAAAAATTTAATCAGTGGAA-3′ | | Sequencing | | 5′-CCACTCCATCGAGATT-3′ |
| | NRAS | 12/13 | F | 5′-AGGTTCTTGCTGGTGTGAAATGAC-3′ | | | R | 5′-B-TGGATTGTCAGTGCGCTTTTC-3′ | | Sequencing | | 5′-TGGTGGTGGTTGGAG-3′ |
| | NRAS | 61 | F | 5′-GATTCTTACAGAAAACAAGTGGTTATAGAT-3′ | | | R | 5′-B-GCAAATACACAGAGGAAGCCTTCG-3′ | | Sequencing | | 5′-GACATACTGGATACAGCTGG-3′ |
| | HRAS | 12/13 | F | 5′-B-AGGAGCGATGACGGAATATAAGC-3′ | | | R | 5′-TCTATAGTGGGGTCGTATTCGTCC-3′ | | Sequencing | | 5′-TCTTGCCCACACCGC-3′ |
| | HRAS | 61 | F | 5′-B-CCTGTTGGACATCCTGGATACC-3′ | | | R | 5′-GTGCGCATGTACTGGTCCC-3′ | | Sequencing | | 5′-CATGGCGCTGTACTC-3′ |
| | KRAS | 12/13 | F | 5′-CTGGTGGAGTATTTGATAGTGTA-3′ | | | R | 5′-B-TGGTCCTGCACCAGTAATAT-3′ | | Sequencing | | 5′-ATAAACTTGTGGTAGTTGG-3′ |
| | KRAS | 61 | F | 5′-B-TCCAGACTGTGTTTCTCCCTTCTC-3′ | | | R | 5′-TACTGGTCCCTCATTGCACTGTAC-3′ | | Sequencing | | 5′-CCTCATTGCACTGTACTC-3′ |
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