The J-Domain of Heat Shock Protein 40 Can Enhance the Transduction Efficiency of Arginine-Rich Cell-Penetrating Peptides

Figure 2

Expression of CPP-DsRed recombinant proteins. (a) Total lysates of E. coli expressing PTD-DsRed, DPV3A-DsRed, pET22b-E162-DsRed, pVEC-DsRed, R11-DsRed, and TP13-DsRed recombinant proteins (left panel) as well as those of PTD-J-DsRed, DPV3-J-DsRed, E162-J-DsRed, pVEC-J-DsRed, R11-J-DsRed, and TP13-J-DsRed recombinant proteins (right panel) were analyzed by SDS-PAGE. The recombinant proteins are indicated by braces. (b) The protein contents of medium fractions from E. coli expressing PTD-DsRed, DPV3A-DsRed, E162-DsRed, pVEC-DsRed, and TP13-DsRed recombinant proteins (left panel) as well as those of PTD-J-DsRed, DPV3-J-DsRed, E162-J-DsRed, pVEC-J-DsRed, R11-J-DsRed, and TP13-J-DsRed (right panel) were analyzed by SDS-PAGE. A 38 kDa common secreted protein OmpF from host cells is indicated by an arrow as internal standard and the recombinant proteins are indicated by braces. (c) The soluble DsRed, J-DsRed, PTD-DsRed, PTD-J-DsRed, DPV3A-DsRed, and DPV3A-J-DsRed recombinant proteins were purified by Ni-Sepharose 6 Fast Flow affinity column chromatography. Each lane was loaded with 20 μg of purified recombinant proteins.

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