BioMed Research International / 2015 / Article / Tab 1

Research Article

The J-Domain of Heat Shock Protein 40 Can Enhance the Transduction Efficiency of Arginine-Rich Cell-Penetrating Peptides

Table 1

Primers for pET22b-CPP vectors construction.

PTDPeptideYGRKK RRQRR R
Forward primerTATG GCT TAT GGT CGT AAG AAA CGT CGT CAG CGT CGT CGT  GTG GGG ATC CCG
Reverse primerAATT CGG GAT CCC CAC  ACG ACG ACG CTG ACG ACG TTT CTT ACG ACC ATA AGC CA

DPV3APeptideAKKRR RESRK KRRRE S
Forward primerTATG GCT AAA AAA CGC CGT CGT GAA AGC CGT AAA AAA CGT CGT CGT GAA AGC GGG ATC CCG
Reverse primerA ATT CGG GAT CCC GCA TTC ACG ACG ACG TTT TTT ACG GCT TTC ACG ACG GCG TTT TTT AGC CA

E162PeptideKTVLL RKLLK LLVRK I
Forward primerTATG AAA ACC GTG CTG CTG CGT AAA CTG CTG AAA CTG CTG GTG CGT AAA ATC GGG ATC CCG
Reverse primerA ATT CGG GAT CCC GAT TTT ACG CAC CAG CAG TTT CAG CAG TTT ACG CAG CAG CAC GGT TTT CA

pVECPeptideLLIIL RRRIR KQAHA HSK
Forward primerTATG CTG CTG ATT ATT CTG CGT CGT CGC ATT CGT AAA CAG GCC CAT GCC CAT TCT AAA GGG ATC CCG
Reverse primerA ATT CGG GAT CCC TTT AGA ATG GGC ATG GGC CTA TTT ACG AAT GCG ACG ACG CAG AAT AAT CAG CAG CA

R11PeptideRRRRR RRRRR R
Forward primerTATG CGC CGT CGT CGT CGC CGT CGT CGC CGT CGT CGT GGG ATC CCG
Reverse primerA ATT CGG GAT CCC ACG ACG ACG GCG ACG ACG GCG ACG ACG ACG GCG CA

TP13PeptideLNSAG YLLGK ALAAL AKKIL
Forward primerTATG CTG AAC AGC GCG GGT TAT CTG CTG GGT AAA GCC CTG GCC GCC CTG GCG AAA AAG ATT CTG GGG ATC CCG
Reverse primerA ATT CGG GAT CCC CAG AAT CTT TTT CGC CAG GGC GGC CAG GGC TTT ACC CAG CAG ATA ACC CGC GCT GTT CAG CA

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