Protective Effects of Cilastatin against Vancomycin-Induced Nephrotoxicity
Effects of cilastatin on vancomycin-treated renal proximal tubular epithelial cells (RPTECs) morphology. RPTECs were cultured in the presence of vancomycin (0.6, 3, and 6 mg/mL) and vancomycin plus cilastatin (200 μg/mL) for 24 hours. (a) Phase-contrast photomicrographs are shown (representative example of at least three independent experiments; original magnification 40x). (b) Effect of cilastatin on vancomycin-induced detachment of RPTECs, measured by flow cytometry and determined by counting the number of cells in an equal volume of buffer. Data are represented as the mean SEM of at least three separate experiments. versus control and control plus cilastatin, versus the same data without cilastatin.