Protective Effects of Cilastatin against Vancomycin-Induced Nephrotoxicity
Effect of cilastatin on vancomycin-induced mitochondrial damage. Renal proximal tubular epithelial cells (RPTECs) were exposed to vancomycin and vancomycin plus cilastatin (200 μg/mL) for 24 hours. (a) Cell viability was determined by the ability to reduce MTT. Results are expressed as the percentage of the value obtained relative to control (without vancomycin and cilastatin) of at least three separate experiments. (b) Changes in the mitochondrial oxidative capacity of RPTECs were assessed by MTT reduction at 570 nm. The graph shows formation of formazan as detected in isolated cells in real time with no treatment (control) and vancomycin 6 mg/mL with or without 200 μg/mL cilastatin, after the incubation times in seconds given on the -axis. versus control and control plus cilastatin, versus the same data without cilastatin.