Protective Effects of Cilastatin against Vancomycin-Induced Nephrotoxicity
Effects of cilastatin on vancomycin accumulation in proximal tubular epithelial cells (RPTECs). Intracellular accumulation was measured in lysates of PTECs treated with vancomycin 0.6, 3, and 6 mg/mL for 24 hours, in the presence or absence of cilastatin (200 μg/mL), using fluorescence polarization immunoassay (TDX) specific assays. Cilastatin was shown to prevent entry of vancomycin into RPTECs. Values were expressed as means SEM of vancomycin concentration ( different experiments). ANOVA model . Factors: cilastatin effect ; dose effect .