Evaluation of Antioxidant and Antibacterial Potentials of Nigella sativa L. Suspension Cultures under Elicitation
Table 5
Zone of inhibition of N. sativa suspension cultures under pectin and MnCl2 elicitation.
S. number
Organism
Diameter of zone of inhibition (mm)
A
B
C
D
E
F
G
H
Antibiotics
ST
CF
DO
AM
OF
1
E. coli
22 ± 0.3
32 ± 0.4
22 ± 0.3
24 ± 0.4
27 ± 0.5
20 ± 0.2
30 ± 0.2
18 ± 0.2
12 ± 0.2
12 ± 0.4
16 ± 0.4
11 ± 0.3
10 ± 0.2
2
S. typhi
21 ± 0.6
28 ± 0.4
18 ± 0.3
20 ± 0.3
20 ± 0.4
18 ± 0.6
28 ± 0.4
16 ± 0.4
16 ± 0.3
11 ± 0.3
0
0
0
3
B. cereus
20 ± 0.7
32 ± 0.6
18 ± 0.4
22 ± 0.3
30 ± 0.7
20 ± 0.3
28 ± 0.2
17 ± 0.8
14 ± 0.2
18 ± 0.4
12 ± 0.2
12 ± 0.4
13 ± 0.4
4
S. aureus
20 ± 0.3
30 ± 0.2
20 ± 0.2
21 ± 0.7
26 ± 0.3
19 ± 0.3
30 ± 0.6
17 ± 0.3
19 ± 0.5
14 ± 0.5
10 ± 0.2
0
0
5
K. pneumoniae
18 ± 0.2
30 ± 0.5
21 ± 0.2
20 ± 0.6
24 ± 0.3
17 ± 0.8
28 ± 0.4
15 ± 0.7
20 ± 0.3
14 ± 0.2
13 ± 0.4
0
15 ± 0.5
A = epicotyl extract; B = control suspension culture; C = pectin 5 mg/L elicitation; D = pectin 10 mg/L elicitation; E = pectin 15 mg/L elicitation; F = MnCl2 5 mg/L elicitation; G = MnCl2 10 mg/L elicitation; H = MnCl2 15 mg/L elicitation; “−” = absent and “+” = present; ST = streptomycin; CF = ciprofloxacin; DO = doxycycline; AM = ampicillin; OF = ofloxacin; and susp. = suspension.
