Effect of Short-Term Stimulation with Interleukin-1β and Differentiation Medium on Human Mesenchymal Stromal Cell Paracrine Activity in Coculture with Osteoblasts

Figure 2

hMSC gene expression. Fold change in relative gene expression of hMSCs normalized to unstimulated hMSCs (RPLP at day 0) quantified by real-time PCR. Bars show the mean ± SEM of 3 different experiments performed in triplicate per group ( per group with the exception of (e) day 7 in CM + IL-1β, where ). D’Agostino-Pearson omnibus normality test was used followed by one-way ANOVA, . (a) Runx2 on days 2 and 7, (b) Runx2 on days 14 and 21, (c) Sox9 on days 2 and 7, (d) Sox9 on days 14 and 21, (e) ALP on days 2 and 7, (f) ALP on days 14 and 21, (g) Type 1 Col on days 2 and 7, (h) Type 1 Col on days 14 and 21, (i) OC on days 2 and 7, and (j) OC on days 14 and 21.

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