Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda

Figure 1

Triplex real-time PCR amplification pattern using the Brucella genus probe. Fluorescence ratio is plotted against the number of PCR cycles to monitor amplification in real-time mode. Isolates with weak Ct values (29.18 and above) had Brucella-like phenotypic characteristics and were included in this assay.