Isolation and Molecular Characterization of Brucella Isolates in Cattle Milk in Uganda
Table 2
Brucella spp. isolates recovered from cattle milk samples in northern and eastern Uganda (2011-2012) and their MLVA 16 profile compared to that of some known Brucella strains from Brucella MLVA database and strains conforming to B. abortus former biovar 7.
Sample ID/strains
Year
Region
Host
Specimen
Number of repeats for the polymorphic panel 1 and panel 2 Bruce loci
Species
Biovar
Genotype
Panel 1
Panel 2A
Panel 2B
6
8
11
12
42
43
45
55
18
19
21
4
7
9
16
30
G41/01
2011
Gulu
Cattle
Milk
2
4
2
12
3
2
3
3
5
9
6
4
2
9
8
4
B. abortus
ND
UG Ba-m
S406/02
2012
Soroti
Cattle
Milk
2
4
2
12
3
2
3
3
5
9
6
4
2
9
8
4
B. abortus
ND
UG Ba-m
S37/02
2012
Soroti
Cattle
Milk
2
4
2
12
3
2
3
3
5
9
6
4
2
9
8
4
B. abortus
ND
UG Ba-m
S03/03
2012
Soroti
Cattle
Milk
2
4
2
12
3
2
3
3
5
9
6
4
2
9
8
4
B. abortus
ND
UG Ba-m
S403/07
2012
Soroti
Cattle
Milk
2
4
2
12
3
2
3
3
5
9
6
4
2
9
8
4
B. abortus
ND
UG Ba-m
S406/01
2012
Soroti
Cattle
Milk
2
4
2
12
3
2
3
3
5
9
6
4
2
9
8
4
B. abortus
ND
UG Ba-m
G86/14
2011
Gulu
Cattle
Milk
2
4
2
12
3
2
3
3
5
9
6
4
2
9
8
4
B. abortus
ND
UG Ba-m
S403/12
2012
Soroti
Cattle
Milk
2
4
2
12
3
2
3
3
5
9
6
4
2
9
8
4
B. abortus
ND
UG Ba-m
S403/09
2012
Soroti
Cattle
Milk
2
4
2
12
3
2
3
3
5
9
6
4
2
9
8
4
B. abortus
ND
UG Ba-m
S09/08
2012
Soroti
Cattle
Milk
2
4
2
12
3
2
3
3
5
9
6
4
2
9
8
4
B. abortus
ND
UG Ba-m
S02/10
2012
Soroti
Cattle
Milk
2
4
2
12
3
2
3
3
5
9
6
4
2
9
8
4
B. abortus
ND
UG Ba-m
*REF 1330
—
USA
Swine
—
2
3
6
10
4
1
5
2
4
38
9
6
6
5
5
3
B. suis
Biovar 1
33 (MLVA-11)
aBCCN#87-92
1997
USA
Human
—
2
4
2
12
4
2
3
3
9
36
7
2
4
3
3
5
B. melitensis
Biovar 1
126 (MLVA-11)
aREF 544
1942
England
Bovine
—
4
5
4
12
2
2
3
3
5
42
8
3
5
3
4
5
B. abortus
Biovar 1
83 (MLVA-11)
aREF 86/8/59
1959
England
Bovine
—
4
5
4
12
2
1
3
3
6
42
8
3
4
3
3
5
B. abortus
Biovar 2
80 (MLVA-11)
aREF Tulya
1958
Uganda
Human
—
3
5
4
11
2
2
3
3
8
40
8
6
5
3
11
5
B. abortus
Biovar 3
64 (MLVA-11)
aREF 292
1961
England
Bovine
—
4
5
4
12
2
2
3
2
6
42
8
3
4
3
3
5
B. abortus
Biovar 4
78 (MLVA-11)
aREF B3196
1959
England
Bovine
—
3
5
3
12
2
2
2
3
7
42
8
6
7
3
3
3
B. abortus
Biovar 5
67 (MLVA-11)
aREF 870
1959
Africa
Bovine
—
3
5
3
12
2
2
3
3
7
42
8
3
6
3
3
3
B. abortus
Biovar 6
66 (MLVA-11)
aREF C68
1958
England
Bovine
—
3
5
3
12
2
2
2
3
7
42
8
6
6
3
3
3
B. abortus
Biovar 9
67 (MLVA-11)
aBCCN#V1 (S19)
1943
USA
Bovine
—
4
5
4
12
2
2
3
3
6
42
8
3
5
3
3
5
B. abortus
Biovar 1
82 (MLVA-11)
aBCCN#V5 (RB51)
—
USA
Bovine
—
4
5
4
12
2
3
3
3
6
42
8
3
7
3
3
5
B. abortus
Biovar 1
79 (MLVA-11)
a13
2006-2007
Egypt
Cattle
Spleen
4
5
4
12
2
3
3
3
6
—
8
3
7
3
3
5
B. abortus
—
—
a4
2002–2007
Egypt
Cattle
—
4
5
4
12
2
3
3
3
6
—
8
3
9
3
3
5
B. abortus
—
—
a6-KEBa 1
2009
Kenya
Cattle
Abortion material
3
5
4
11
2
2
3
3
7
40
8
6
6
3
11
6
B. abortus
Biovar 3
34 (MLVA-8)
a11-KEBa 2
2009
Kenya
Cattle
Vaginal discharge
3
5
4
11
2
2
3
3
7
40
8
6
5
3
12
5
B. abortus
Biovar 3
34 (MLVA-8)
aBCCN#93-26
1993
Sudan
Dromedary
—
3
5
4
11
2
2
3
3
6
40
8
6
8
3
7
7
B. abortus
Biovar 3
63 (MLVA-11)
b07-994-2411
1963
Kenya
Bovine
—
2
4
2
12
3
2
3
3
5
22
6
5
2
6
7
5
B. abortus
ND
ND
b03-4923-239
2003
Turkey
Bovine
—
4
5
3
12
2
2
3
1
6
21
8
6
7
6
3
3
B. abortus
ND
ND
b99-9971-135
1988
Mongolia
Bovine
—
4
5
5
12
2
2
2
2
6
21
8
5
6
4
3
3
B. abortus
ND
ND
b99-9971-159
1993
Mongolia
Bovine
—
4
5
5
12
2
2
2
2
6
21
8
5
6
4
3
3
B. abortus
ND
ND
Strains downloaded from Brucella MLVA database February 2014; http://mlva.u-psud.fr; *the reference strain used (from Bruce-ladder kit); ND: not designated; bstrains conforming to B. abortus former biovar 7.
