β-Catenin-Dependent Signaling Pathway Contributes to Renal Fibrosis in Hypertensive Rats

Figure 10

Effect of pyrvinium pamoate on the levels of OPN and fibronectin in 2K1C hypertensive rats. Unclipped kidneys from 2K1C rats treated with pyrvinium pamoate were immunostained for OPN and the levels of fibronectin were evaluated by Western blot. (a) Sham, (b) 2K1C rats, and (c) 2K1C rats treated with pyrvinium pamoate. (d) Quantification of OPN IF. (e) Western blot of fibronectin protein levels normalized to β-actin levels. Numbers (1, 2, 3, and 4) in the Western blot indicate an individual animal sample in a given group. The sections from unclipped kidney from 2K1C hypertensive rats treated with pyrvinium pamoate show a reduction of both immunofluorescence of OPN and fibronectin protein level in total kidney extracts. Scale bar = 100 μm. The values represent the mean ± SEM (). .

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