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BioMed Research International
Volume 2015 (2015), Article ID 727304, 7 pages
http://dx.doi.org/10.1155/2015/727304
Research Article

Antimicrobial, Antioxidant, and Cytotoxic Properties of Vasicine Acetate Synthesized from Vasicine Isolated from Adhatoda vasica L.

1Department of Botany and Microbiology, Addiriyah Chair for Environmental Studies, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia
2Division of Microbiology, Entomology Research Institute, Loyola College, Chennai 600034, India
3Visiting Professor Programme, Deanship of Scientific Research, College of Science, King Saud University, Saudi Arabia

Received 11 May 2014; Revised 28 September 2014; Accepted 7 October 2014

Academic Editor: Paul M. Tulkens

Copyright © 2015 V. Duraipandiyan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Adhatoda vasica (L.) (Acanthaceae) is used in the indigenous system of medicine in India. The alkaloid Vasicine was isolated from ethanolic extract of the leaves of A. vasica using column chromatography. Vasicine acetate was obtained by acetylation of Vasicine. Vasicine acetate exhibited good zone of inhibition against bacteria: 10 mm against E. aerogenes, 10 mm against S. epidermidis, and 10 mm against P. aeruginosa. Vasicine acetate showed minimum inhibitory concentration values against bacteria: M. luteus (125 μg/mL), E. aerogenes (125 μg/mL), S. epidermidis (125 μg/mL), and P. aeruginosa (125 μg/mL). The radical scavenging activity of Vasicine acetate was the maximum at 1000 μg/mL (66.15%). The compound showed prominent cytotoxic activity in vitro against A549 lung adenocarcinoma cancer cell line. Quantification of Vasicine and Vasicine acetate by HPLC-DAD analysis showed their contents to be 0.2293% and 0.0156%, respectively, on dry weight basis of the leaves. Vasicine acetate could be probed further in drug discovery programme.