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BioMed Research International
Volume 2015, Article ID 730390, 8 pages
Research Article

Expression of Translationally Controlled Tumor Protein in Human Kidney and in Renal Cell Carcinoma

1Section of Pathology, Department of Medical Biotechnology, University of Siena, Via delle Scotte 6, 53100 Siena, Italy
2Section of Pathology, Ospedale di Circolo di Busto Arsizio, Presidio Ospedaliero di Saronno, Piazzale Borella 1, 21047 Saronno, Italy
3Section of Pathology, Azienda Ospedaliera, “G. Salvini”, Viale C. Forlanini 121, 20024 Garbagnate Milanese, Italy
4School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London E14NS, UK
5Department of Medicine, Science and Neurosciences, University of Siena, Via delle Scotte 6, 53100 Siena, Italy
6Section of Pathology, Azienda Ospedaliera Universitaria Senese, Viale Bracci 16, 53100 Siena, Italy

Received 25 February 2015; Revised 1 June 2015; Accepted 10 June 2015

Academic Editor: Kong Chuize

Copyright © 2015 Maria R. Ambrosio et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Translationally controlled tumor protein is a multifaceted protein involved in several physiological and biological functions. Its expression in normal kidney and in renal carcinomas, once corroborated by functional data, may add elements to elucidate renal physiology and carcinogenesis. In this study, translationally controlled tumor protein expression was evaluated by quantitative real time polymerase chain reaction and western blotting, and its localization was examined by immunohistochemistry on 84 nephrectomies for cancer. In normal kidney protein expression was found in the cytoplasm of proximal and distal tubular cells, in cells of the thick segment of the loop of Henle, and in urothelial cells of the pelvis. It was also detectable in cells of renal carcinoma with different pattern of localization (membranous and cytoplasmic) depending on tumor histotype. Our data may suggest an involvement of translationally controlled tumor protein in normal physiology and carcinogenesis. However, functional in vitro and in vivo studies are needed to verify this hypothesis.