Advanced Glycation End Products Enhance Macrophages Polarization into M1 Phenotype through Activating RAGE/NF-κB Pathway

Figure 3

Effects of AGEs on mRNA levels and expressions of iNOS and Arg1. For mRNA evaluation, the cells were cultured with different concentrations of AGEs for 24 h. For protein expression detection, the cells were incubated with AGEs 500 μg/mL for 24 h. BSA 500 μg/mL was used as a control. The mRNA levels were assayed by real time PCR, and protein expression was measured by FCM. AGEs significantly upregulated iNOS mRNA expression in a dose dependent manner (a) but did not affect Arg1 mRNA level (b). AGEs increased iNOS expression (c) but did not affect the expression of Arg1 (d). ; ; and compared with control. MFI: mean fluorescence intensity. Each bar represents the mean value ± SD of triplicate determinations, representative of three independent experiments.

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