Advanced Glycation End Products Enhance Macrophages Polarization into M1 Phenotype through Activating RAGE/NF-κB Pathway

Figure 6

Role of AGEs on RAGE/NF-κB pathway. The cells were incubated with AGEs 500 μg/mL for different time. The p-p65 and total p65 protein were measured as MFI (mean fluorescence intensity) by FCM. The NF-κB pathway was activated by AGEs (a). After pretreatment with anti-RAGE antibody for 60 min, NF-κB was still activated by AGEs (b). NF-κB pathway activation was partly inhibited by anti-RAGE antibody (c). Each point represents the mean value ± SD of triplicate determinations, representative of three independent experiments.

(a)

(b)

(c)