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BioMed Research International
Volume 2015 (2015), Article ID 732573, 9 pages
http://dx.doi.org/10.1155/2015/732573
Research Article

Development of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Trichosporon asahii in Experimental and Clinical Samples

1Department of Dermatology, General Hospital of Beijing Military Command of PLA, No.5, Nanmencang, Dongcheng District, Beijing 100010, China
2The Clinical Medical College in the Beijing Military Region of Second Military Medical University of PLA, Beijing 100700, China

Received 18 September 2014; Accepted 2 January 2015

Academic Editor: Mansour El-Matbouli

Copyright © 2015 Jianfeng Zhou et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Invasive trichosporonosis is a deep mycosis found mainly in immunocompromised hosts, and the major pathogen is Trichosporon asahii. We detected the species-specific intergenic spacers (IGS) of rRNA gene of T. asahii using a loop-mediated isothermal amplification (LAMP) assay in 15 isolates with 3 different visualization methods, including SYBR green detection, gel electrophoresis, and turbidimetric methods. The LAMP assay displayed superior rapidity to other traditional methods in the detection time; that is, only 1 h was needed for detection and identification of the pathogen DNA. Furthermore, the detection limit of the LAMP assay was more sensitive than the PCR assay. We also successfully detect the presence of T. asahii in samples from experimentally infected mice and samples from patients with invasive trichosporonosis caused by T. asahii, suggesting that this method may become useful in clinical applications in the near future.