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BioMed Research International
Volume 2015, Article ID 738241, 13 pages
Research Article

Heteronemin, a Spongean Sesterterpene, Induces Cell Apoptosis and Autophagy in Human Renal Carcinoma Cells

1Pharmacological Institute, College of Medicine, National Taiwan University, No. 1 Jen-Ai Road, Section 1, Taipei 10051, Taiwan
2Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944, Taiwan
3National Museum of Marine Biology and Aquarium, Pingtung 944, Taiwan
4The Ph.D. Program for Cancer Biology and Drug Discovery, College of Medical Science and Technology, Taipei Medical University, No. 250 Wu-Hsing Street, Taipei 11031, Taiwan
5Department of Pharmacology, College of Medicine, Taipei Medical University, No. 250 Wu-Hsing Street, Taipei 110311, Taiwan

Received 23 July 2014; Accepted 26 August 2014

Academic Editor: Chin-Chung Wu

Copyright © 2015 Szu-Ying Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure S1: Effects of heteronemin on cell cycle distribution in A498 cells. Cells were incubated with (A) DMSO or various concentrations of heteronemin for 24 h and (B) DMSO or 3 μM heteronemin for the indicated time periods. Cell cycle phase and cell apoptosis were determined by FACS as described in Materials and Methods.

Supplementary Figure S2: Effects of SB203580 on heteronemin-induced LC3 conversion in A498 cells. Cells were incubated with DMSO, heteronemin 3 μM, SB203580 25 μM and combination of heteronemin and SB203580 for 24 h and detected protein expression by western blotting. DMSO was used as the vehicle control (CTL).

  1. Supplementary Figures