Nucleofection of Rat Pheochromocytoma PC-12 Cells with Human Mutated Beta-Amyloid Precursor Protein Gene (APP-sw) Leads to Reduced Viability, Autophagy-Like Process, and Increased Expression and Secretion of Beta Amyloid

Figure 1

Bar charts (means ± SEM) represent cell viability (MTT assay, upper panel) or Aβ 1-40 concentration in supernatants (HTRF assay, lower panel) normalized for cell viability as determined with the MTT assay measured in the same wells of multiwell plates. Values are expressed as % of control (untreated PC-12 cells nucleofected with GFP, or GFP + APP-wt, or GFP + APP-sw). ((a)–(c)) The effect of atorvastatin (10 μM), simvastatin (10 μM), or MβCD (0.2 mM); ((b)–(d)) the effect of TNF-α (10 ng/mL), TRAIL (10 ng/mL), or nystatin (1 μM). Different lower case letters indicate statistically significant differences between means ().

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