Isolation and Characterization of Human Lung Lymphatic Endothelial Cells

Figure 4

Detection of Receptor Tyrosine Kinases on HL-LECs (P4). (a) and (b) (immunofluorescence): green fluorescence corresponds to the surface expression of vascular endothelial growth factor receptor- (VEGFR-) 2 and VEGFR-3, respectively, in cultured HL-LECs. (c): representative image of a negative control in which the primary antibody was omitted from the reaction. Nuclei are shown by the blue fluorescence of DAPI. (d)–(j) (immunoperoxidase): the brownish precipitate on the surface of HL-LECs on each panel corresponds respectively to the expression of Platelet-Derived Growth Factor Receptor- (PDGFR-) beta, Epidermal Growth Factor Receptor- (EGFR-) 1, Fibroblast Growth Factor Receptor- (FGFR-) 1, type 1 Insulin-like Growth Factor Receptor (IGF-1R), the Hepatocyte Growth Factor receptor c-MET, Tropomyosin-related kinases A (TrkA), and neurotrophin p75 receptor (). (k): representative image of a negative control in which the primary antibody was omitted from the reaction. Nuclei are counterstained with Haematoxylin. Scale bars: (a), (d), (e), (f), (g), (h), (i), (j), and (k) = 50 μm; (b) and (c) = 250 μm.

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