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BioMed Research International
Volume 2015, Article ID 748121, 10 pages
Research Article

Hypergravity Stimulation Enhances PC12 Neuron-Like Cell Differentiation

1Istituto Italiano di Tecnologia, Center for Micro-BioRobotics @SSSA, Viale Rinaldo Piaggio 34, 56025 Pontedera (Pisa), Italy
2ASAcampus Joint Laboratory, ASA Research Division, Department of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy

Received 4 November 2014; Revised 8 January 2015; Accepted 27 January 2015

Academic Editor: Aijun Wang

Copyright © 2015 Giada Graziana Genchi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1. reports additional fluorescence microscopy images (ß3-tubulin immunostaining) taken at low magnification to show PC12 cell neurite outgrowth and extension after hypergravity treatments.

Figure S2. depicts the distributions of neurite lengths measured on different samples exposed to hypergravity.

Figure S3. shows additional low magnification immunofluorescence microscopy images of PC12 cells presenting different localization of the differentiation marker neurofilament-66 after hypergravity treatments.

  1. Supplementary Figures