BioMed Research International / 2015 / Article / Fig 4

Research Article

Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures

Figure 4

(a) Mesencephalic cultures were first established for 7 days and incubated with cocaine (1.0 mM) for 24 hours. After cells were fixed, the TUNEL method was used. Cultures were photographed at the level of the neuronal layer. Note the labeling in the vast majority of treated cells, in contrast with the labeling of a few control cells. TUNEL positive cells were dUTP labeled (brown label). The neurons were labeled with MAP2 and NeuN (green label) and Hoechst 33342 (blue label) was added to monitor chromatin condensation. (b) Number of TUNEL positive cells observed by immunostaining of the mesencephalic primary culture treated with PBS or cocaine for 24 hours. Values are mean ± SEM from five independent experiments. Cocaine treatment decreased neuronal viability. Significantly different from the control (PBS) value: by Student’s t-test.
(a)
(b)

We are committed to sharing findings related to COVID-19 as quickly as possible. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. Review articles are excluded from this waiver policy. Sign up here as a reviewer to help fast-track new submissions.