Downregulation of miR-221 Inhibits Cell Migration and Invasion through Targeting Methyl-CpG Binding Domain Protein 2 in Human Oral Squamous Cell Carcinoma Cells

Figure 4

MBD2 is a direct target of miR-221. (a) Predicted duplex formation between the wild-type or mutant MBD2 3′-UTR and miR-221. (b) Western blot showing MBD2 protein expression level in OSCC cell lines. Actin was used as an internal loading control. (c) Luciferase activity of wild-type (3′-UTR-wild) or mutant (3′-UTR-mutant) MBD2 3′-UTR-containing reporters in UM1 cells transfected with miR-221 mimic or miR-NC. (d) qRT-PCR of MBD2 mRNA in UM1 cells transfected with miR-221 mimic or miR-NC. Data were normalized to GAPDH mRNA. Data are expressed as mean ± SD; . (e) Western blot of MBD2 in UM1 cells transfected with miR-221 mimic or miR-NC. Actin was used as an internal loading control.

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