Research Article

RCCS Bioreactor-Based Modelled Microgravity Induces Significant Changes on In Vitro 3D Neuroglial Cell Cultures

Figure 4

Neuronal marker localisation in SH-SY5Y cells. Representative confocal images of SH-SY5Y cells cultured as a monolayer (2D, (a), (c), and (e)) and under the modelled microgravity (RCCS bioreactor, (b), (d), and (f)) and immunostained with anti-N-CAM ((a) and (b)), anti-GAP43 ((c) and (d)), and anti-tyrosine hydroxylase (TH) ((e) and (f)) antibodies (as indicated). All of the cells were also stained with propidium iodide (PI). Insets show image magnification. Scale bars, 20 μm.