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BioMed Research International
Volume 2015, Article ID 756359, 6 pages
http://dx.doi.org/10.1155/2015/756359
Research Article

A Pyrosequencing Assay for the Quantitative Methylation Analysis of GALR1 in Endometrial Samples: Preliminary Results

1Department of Cytopathology, “ATTIKON” University Hospital, University of Athens Medical School, 1 Rimini, Haidari, 12462 Athens, Greece
22nd Department of Gynecology, St. Savas Cancer Hospital, 171 Alexandras Avenue, 11522 Athens, Greece
3Department of Obstetrics and Gynecology, IASO Hospital, 37-39 Kifisias Avenue, Maroussi, 15123 Athens, Greece
43rd Department of Obstetrics and Gynecology, “ATTIKON” University Hospital, 1 Rimini, Haidari, 12462 Athens, Greece
52nd Department of Pathology, “ATTIKON” University Hospital, University of Athens Medical School, 1 Rimini, Haidari, 12462 Athens, Greece

Received 26 March 2015; Revised 3 June 2015; Accepted 4 June 2015

Academic Editor: Maria Lina Tornesello

Copyright © 2015 Christine Kottaridi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Endometrial cancer is the most common malignancy of the female genital tract while aberrant DNA methylation seems to play a critical role in endometrial carcinogenesis. Galanin’s expression has been involved in many cancers. We developed a new pyrosequencing assay that quantifies DNA methylation of galanin’s receptor-1 (GALR1). In this study, the preliminary results indicate that pyrosequencing methylation analysis of GALR1 promoter can be a useful ancillary marker to cytology as the histological status can successfully predict. This marker has the potential to lead towards better management of women with endometrial lesions and eventually reduce unnecessary interventions. In addition it can provide early warning for women with negative cytological result.