Extracellular Calcium-Dependent Modulation of Endothelium Relaxation in Rat Mesenteric Small Artery: The Role of Potassium Signaling

Figure 5

Incubation with 10 μM ouabain in the presence of 100 μM L-NAME and 3 μM indomethacin suppressed ACh-dependent relaxations. The concentration-response curves were constructed for the experiments in the bath solutions containing 4.2 mM ((a) ) and 5.9 mM ((b) ) . (c) shows the areas under curves (AUC) from (a) and (b). The changes in sensitivities to ACh (pD₂) before and after incubation are shown in (d). The changes in maximal relaxations (to 10 μM ACh) before and after the treatment with ouabain are shown in (e). and indicate and 0.001 for the responses before and after incubation with ouabain. ## and ### indicate and 0.001 for 4.2 mM versus 5.9 mM .

(a)

(b)

(c)

(d)

(e)