Magnetic Resonance Imaging of Atherosclerosis Using CD81-Targeted Microparticles of Iron Oxide in Mice

Figure 2

Confocal laser scanning microscopy shows CD81-MPIO binding to bEnd.3 cells. The cells were stimulated with 5 μM PMS for 16 h and incubated with FITC-labeled CD81-MPIO or IgG-MPIO. After that, the cells were stained by DAPI and DiI dyes and examined under confocal laser scanning microscopy. (a) The MPIO particles appeared green. (b) The nuclei showed blue. (c) The cell membrane was stained red. (d) The merged image from (a), (b), and (c) showed CD81-MPIO can bind to PMS-stimulated cells. (e–h) As a control, IgG-MPIO did not bind to the PMS-stimulated bEnd.3 cells (bar = 20 μm).

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