(a)
(b)
(c)
(d)
Figure 5: LNG increases connexin 43 protein levels and does not affect the level of p-connexin 43 at the S368 site, as measured by western blot analysis. HESCs were treated with either 5 × 10−5 mol/L LNG or the negative control (0.5% DMSO) for 24 hs, 48 hs, 72 hs, and 96 hs; then the protein lysis was measured by western blot (). ((a), (b)) Expression of total connexin 43 (Cx43) (a) and p-S368 Cx43 (b) treated with 5 × 10−5 mol/L LNG in a time-dependent manner; ((c), (d)) Comparison of the expression of total Cx43 (c) and p-S368 Cx43 (d) treated with 5 × 10−5 mol/L LNG; ; there was a significant difference.