Vitamin A-Deficient Diet Accelerated Atherogenesis in Apolipoprotein E<sup>−/−</sup> Mice and Dietary <i>β</i>-Carotene Prevents This Consequence

Relevy, Noa Zolberg; Harats, Dror; Harari, Ayelet; Ben-Amotz, Ami; Bitzur, Rafael; Rühl, Ralph; Shaish, Aviv

doi:https://doi.org/10.1155/2015/758723

BioMed Research International

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Research Article | Open Access

Volume 2015 | Article ID 758723 | https://doi.org/10.1155/2015/758723

Vitamin A-Deficient Diet Accelerated Atherogenesis in Apolipoprotein E^−/− Mice and Dietary β-Carotene Prevents This Consequence

Noa Zolberg Relevy,^1,2Dror Harats,^1,2Ayelet Harari,¹Ami Ben-Amotz,³Rafael Bitzur,^1,2Ralph Rühl,⁴and Aviv Shaish¹

Academic Editor: Giuseppe Valacchi

Received01 Jul 2014

Revised25 Nov 2014

Accepted03 Dec 2014

Published23 Feb 2015

Abstract

Vitamin A is involved in regulation of glucose concentrations, lipid metabolism, and inflammation, which are major risk factors for atherogenesis. However, the effect of vitamin A deficiency on atherogenesis has not been investigated. Therefore, the objective of the current study was to examine whether vitamin A deficiency accelerates atherogenesis in apolipoprotein E-deficient mice (apoE^−/−). ApoE^−/− mice were allocated into the following groups: control, fed vitamin A-containing chow diet; BC, fed chow diet fortified with Dunaliella powder containing βc isomers; VAD, fed vitamin A-deficient diet; and VAD-BC group, fed vitamin A-deficient diet fortified with a Dunaliella powder. Following 15 weeks of treatment, liver retinol concentration had decreased significantly in the VAD group to about 30% that of control group. Vitamin A-deficient diet significantly increased both plasma cholesterol concentrations and the atherosclerotic lesion area at the aortic sinus (+61%) compared to the control group. Dietary βc fortification inhibited the elevation in plasma cholesterol and retarded atherogenesis in mice fed the vitamin A-deficient diet. The results imply that dietary vitamin A deficiency should be examined as a risk factor for atherosclerosis and that dietary βc, as a sole source of retinoids, can compensate for vitamin A deficiency.

1. Introduction

Vitamin A is involved in regulation of glucose concentrations, lipid metabolism, and inflammation, which are major risk factors for atherosclerosis development [1]. All-trans retinoic acid (RA), the endogenous ligand of the retinoic acid receptors (RARs), inhibited experimental atherosclerosis [2, 3] and increased the process of reverse cholesterol transport from macrophages [4]. In previous studies, we demonstrated that feed fortification with β-carotene, a vitamin A precursor, can inhibit atherosclerosis in mouse models [5, 6]. Nonetheless, the effect of dietary vitamin A deficiency on atherogenesis has not been studied.

The dietary sources of vitamin A are retinol and mainly retinyl esters from animal origin or provitamin A carotenoids from plant sources comprising numerous isomers of β-carotene [7]. Vitamin A deficiency can lead to night blindness, abnormal embryonic development, and various pathological conditions [8]. The major cause of deficiency is low dietary intake of vitamin A, common in developing countries [9]. Several other conditions, such as inflammatory bowel disease, recurrent pancreatitis, excessive alcohol consumption, iron deficiency, and mutation in the β-carotene 15,15′ monooxygenase 1 (BCMO1) gene can also cause vitamin A deficiency [10–13].

Dietary vitamin A (retinol, retinyl esters, and provitamin A carotenoids) is transported in chylomicrons to the liver and extrahepatic tissues following intestinal absorption. It is important to note that 66–75% of vitamin A is transported to the liver and stored in hepatic stellate cells (HSCs), while 25–33% is transported directly to various extrahepatic tissues [14]. The liver secretes retinol bound to retinol-binding protein 4 (RBP4) in order to meet tissue needs [15]. In addition, plasma all-trans RA can also contribute to tissue RA pools [16]. Although retinol bound to RBP4 is the main source of extrahepatic tissues, dietary vitamin A can compensate for the absence of RBP4 in both mice and humans [17, 18].

The effect of dietary vitamin A deficiency on plasma lipid profiles has been investigated. In one study, a vitamin A-deficient diet decreased HDL cholesterol and had no effect on non-HDL cholesterol or on triglyceride concentrations in rats fed with this diet for three months [19], while in a second study, a vitamin A-deficient diet decreased both HDL cholesterol and triglycerides [20]. To our knowledge, there is no data regarding the effect of a vitamin A-deficient diet in animals challenged with a high-cholesterol high-fat diet or in animal models for atherosclerosis.

A recent study demonstrated that BCMO1 deficiency in mice prevents the conversion of β-carotene to retinoids and leads to impaired lipid metabolism [21]. We previously showed that a diet enriched in 9-cis-β-carotene (9CβC) provided as powder of the alga Dunaliella bardawil significantly inhibits atherogenesis in a mouse model [6]. These findings led us to examine whether a vitamin A-deficient diet may accelerate atherogenesis and whether dietary carotenoids as a sole source of vitamin A could compensate for dietary vitamin A deficiency.

2. Materials and Methods

2.1. Mice

Male, 12-week-old apoE-deficient mice (apoE^−/−) (C57BL6 background, Jackson Laboratories) were used. Mice were housed in plastic cages on a 12 h light/12 h dark cycle with free access to feed and water. Mice were killed with isoflurane. The Animal Care and Use Committee of Sheba Medical Center, Tel-Hashomer, approved all animal protocols (682/11).

2.2. Diet

Two commercial diets were used: a normal, low-fat diet (18% protein, 5% fat; TD2018, Harlan Teklad) containing 15 IU/g vitamin A (4.5 µg retinol/g) and a vitamin A-deficient diet (17.7% protein, 5% fat; TD86143, Harlan Teklad). We used a powder of the alga Dunaliella bardawil (a gift from Nikken Sohonsha, Japan) as a source for natural carotenoids. The algal powder contains 6% -carotene (weight/weight), comprised of 50% all-trans and 50% 9-cis isomers [22]. To prepare the feed, 0.25 L of distilled hot water was mixed with 14 g of gelatin until the solution was clear. Then, 1 kg of powdered feed and Dunaliella powder (80 g/kg feed) were thoroughly mixed with the warm gelatin solution. After solidifying, the feed was divided into tablets and stored in the freezer. Feed was replaced every other day to minimize oxidation and degradation of its ingredients.

2.3. Study Design

Experiment 1. Sixty-eight 12-week-old apoE^−/− male mice (weight 25 gr) were allocated into 4 groups, 17 animals per group, according to their body weight, plasma cholesterol, and triglyceride (TG) concentrations. The mice were fed for 15 weeks with the specified diet. The control group was fed normal vitamin A-containing chow diet. The BC group was fed chow diet fortified with the alga Dunaliella. The VAD group was fed a vitamin A-deficient diet, and the VAD-BC group was fed a vitamin A-deficient diet fortified with natural carotenoids of the alga Dunaliella (80 g powder mixed with 1 kg feed). Mice were killed after 15 weeks.

Experiment 2. Fourteen 12-week-old apoE^−/− male mice (weight 25 gr) were allocated into two groups with seven animals per group; the control group was fed chow diet and the VAD group was fed a vitamin A-deficient diet. The mice were fed for 15 weeks with the specified diet and were killed after 15 weeks.

2.4. Lipid Analysis

We used a colorimetric enzymatic procedure to measure total plasma cholesterol (Chol, Roche/Hitachi, Roche Diagnostics) and TG (triglyceride liquid, Senitinel).

2.5. Carotenoid Analysis

BC isomer concentrations in the plasma and in the liver were determined by HPLC according to the method described by Shaish et al. [23].

2.6. Retinol Analysis

Retinol extraction from liver and plasma was carried out the same way as β-carotene extraction. Briefly, samples (~250 mg liver and 200 L plasma) were extracted with 2 mL of ethanol containing 10 µM butylated hydroxytoluene. After the addition of 2 mL of hexane and 1 mL of DDW, the samples were mixed and centrifuged for 5 min at 1000 g. The hexane layers of plasma samples were dried under a stream of N₂. The hexane layers of the liver samples were saponified with 2% KOH in Absolute ETOH for 30 min at 50°C. Then, by adding 2 mL of saline and centrifugation, the hexane layer was removed and dried under a stream of N₂. Dried samples were suspended in 200 µL methanol, and retinol concentrations were determined by reverse phase HPLC on a Vydac C18 column (201TP-54, 250 × 5 mm, 5 μm particle size; Vydac, Hesperia, CA) with methanol/butanol/water 10 mM ammonium acetate as the mobile phase at a flow rate of 0.8 mL/min. Retinol was detected by monitoring its absorbance at 325 nm and by comparison with the retention times of authentic standards. Results are expressed as nanomoles of retinol per gram wet weight of tissue.

2.7. Retinoic Acid Analysis

Concentrations of RAs were determined in mouse plasma by our LC-MS method [24]. In summary, 100 μL plasma was diluted with a threefold volume of isopropanol, vortexed for 10 seconds, put in an ultrasonic bath for 5 minutes, shaken for 6 minutes, and centrifuged at 13,000 rpm in a Heraeus BIOFUGE Fresco at +4°C. After centrifugation, the supernatants were dried in an Eppendorf concentrator 5301 (Eppendorf, Germany) at 30°C. The dried extracts were resuspended with 60 μL of methanol, vortexed, shaken, diluted with 40 μL of 60 mM aqueous ammonium acetate solution, transferred into the autosampler, and subsequently analyzed.

2.8. Isolation of Primary Mouse Hepatic Stellate Cells

Mouse livers were collected and washed three times in isotonic saline. Then, HSCs were isolated using Pronase-Collagenase-DNAse lysate followed by OptiPrep (Sigma-Aldrich) density gradient centrifugation as described previously [25].

2.9. Glucose Tolerance Test

After 15 weeks, a glucose tolerance test (GTT) was performed on mice that were fasted for 4 hours. Mice were injected ip with 20% D-glucose, 1% of body weight (v/w). Blood glucose concentrations were measured periodically up to 120 minutes.

2.10. Assessment of Atherosclerosis in the Aortic Sinus

Atherosclerotic fatty streak lesions were quantified by calculating the lesion areas in the aortic sinus [26].

2.11. Fast Protein Liquid Chromatography Analysis of Lipoproteins

Plasma from 5 mice was pooled and serum lipoproteins were separated by size exclusion chromatography using a Superose-6 column (30 cm) on fast protein liquid chromatography [27].

2.12. Analysis of Gene Expression by Real-Time PCR

A Nucleospin RNA II kit (Macherey-Nagel) was used for RNA extraction. A high capacity cDNA synthesis kit (Applied Biosystems) was used to perform cDNA synthesis. Quantitative real-time PCR was performed with a 7900HT PCR machine (Applied Biosystems), FastStart Universal Probe Master ROX (Roche), and a FAM-labeled TaqMan primer and probe (Applied Biosystems and Roche) for mouse Cyp7α (Mm00484152_mL, Applied Biosystems), Pparγ (Mm01184322_mL, Applied Biosystems), Cyp26a1 (312454, Roche), and Hmgr (Mm01282499_mL, Applied Biosystems). We used Gapdh (307884, Roche) as a reference gene.

2.13. Statistical Analyses

One-way ANOVA was used to compare the treatment effect on atherogenesis, with the post hoc Tukey method used for multiple pairwise comparisons. Repeated measures ANOVA was applied to compare changes in weight gain between the treatment groups over the study period. Significance was considered as . Values in the text are means ± SE. All statistics were conducted using SPSS (release 12.0 SPSS Inc.).

3. Results

The aim of the study was to investigate whether a vitamin A-deficient diet accelerates atherogenesis in apoE^−/− mice and to examine whether fortification of this diet with a Dunaliella powder rich in β-carotene isomers, as a sole source of dietary provitamin A, would compensate for dietary vitamin A deficiency. We placed 68 apoE^−/− male mice (Exp. 1) for 15 weeks on the following diet regimes: chow diet (control); Dunaliella powder-fortified diet, as a natural source for β-carotene isomers (BC), vitamin A-deficient diet (VAD), and vitamin A-deficient diet fortified with Dunaliella powder (VAD-BC). Vitamin A deficiency did not significantly affect weight gain, although a trend towards lower weight was noted in the VAD group (), while weight gain in the BC-fortified groups was similar to the control group (Figure 1).

3.1. Vitamin A-Deficient Diet Reduced Liver Retinol Content

Similar to our previous mouse study [6], administration of Dunaliella powder rich in β-carotene isomers resulted in accumulation of both 9-cis and all-trans-β-carotene isomers in mouse livers. The concentrations of the two isomers were 2-3 times higher in the VAD-BC group compared to the concentrations in the BC group, which also received vitamin A in the form of retinyl ester in the feed (Figure 2(c)). To study whether administration of a vitamin A-deficient diet for 15 weeks led to the vitamin A deficiency in the plasma and liver, we measured retinol concentrations by HPLC in these tissues. Despite the long treatment time of 15 weeks, plasma retinol concentrations were similar in all treatment groups, apparently due to homeostatic regulation (Figure 2(a)). In contrast, liver total retinol concentration was decreased significantly in the VAD group to about 30% that of the control group, indicating that a substantial reservoir of vitamin A still exists (Figure 2(b)). It is important to note that fortification with Dunaliella powder rich in β-carotene isomers did not restore liver retinol, and only a trend toward higher concentrations was detected compared to the mice treated with a vitamin A-deficient diet.

(a)

(b)

(c)

(d)

Figure 2

Plasma and liver retinol and β-carotene concentrations. Plasma retinol concentrations were similar in all groups (a), liver retinol concentration decreased significantly in VAD group (b), liver βc isomer concentrations were significantly higher in VAD-BC group compared to BC group (c), and serum all-trans RA concentrations were similar in all groups (d). Retinol, βc, and RA were analyzed following 15 weeks of treatments (Exp. 1). Values are means ± SE, = 5–8. Within each graph, means without a common letter differ, . Dunaliella treated group (BC); vitamin A-deficient diet group (VAD); vitamin A-deficient diet fortified with Dunaliella group (VAD-BC); retinoic acid (RA).

As most liver vitamin A is stored in hepatic stellate cells, we measured retinol concentrations in isolated stellate cells from control and VAD mice (Exp. 2). As expected, a 50% decline in stellate cell retinol content was detected, 12.9 ng retinol per 10⁶ HSC and 6.7 ng retinol per 10⁶ HSC, respectively.

RA in serum was detected by LC-MS. As RA is one of the vitamin A active derivatives, we assumed that RA concentration would change with respect to diets. In contrast to our assumption that the VAD would reduce and the β-carotene would increase serum all-trans RA concentrations, all-trans RA concentrations were comparable in all examined groups (Figure 2(d)). Moreover, despite the high dose of 9cβC in the diet, 9-cis RA was not detected in any of the four groups.

3.2. Vitamin A-Deficient Diet Increased Plasma Cholesterol Concentrations and Accelerated Atherosclerosis While Dietary β-Carotene Reversed These Effects

Vitamin A-deficient diet significantly increased plasma cholesterol concentrations and FPLC analysis showed that this increase is due to higher concentrations of the atherogenic, non-HDL cholesterol. Dietary β-carotene fortification suppressed this elevation, and plasma cholesterol concentrations in the BC and VAD-BC groups were lower throughout the experiments compared to the control or the VAD group, respectively (Figure 3). The atherosclerotic lesion area was quantified at the aortic sinus by oil-red O staining of the lipids. The red color indicates the presence of atherosclerotic lesions (Figure 5(b)). The vitamin A-deficient diet significantly increased (61%) the atherosclerotic lesion area at the aortic sinus compared to the control group (Figure 4). Remarkably, although β-carotene did not restore the liver retinol pool, it significantly inhibited atherogenesis in mice fed a vitamin A-deficient diet, and the lesion area was similar to the control group fed the vitamin A-containing diet.

(a)

(b)

Figure 3

Plasma cholesterol concentrations. Plasma cholesterol concentrations (a) and lipoprotein cholesterol content (b). Mice fed chow or vitamin A-deficient diet with or without βC after 15 weeks of treatment. VAD significantly increased plasma cholesterol concentrations due to increased levels of non-HDL cholesterol. Values are means ± SE, = 15-16. Within the graph, means without a common letter differ, . Dunaliella treated group (BC); vitamin A-deficient diet group (VAD); vitamin A-deficient diet fortified with Dunaliella group (VAD-BC).

(a)

(b)

Figure 4

Atherosclerotic lesion area in apoE^−/− mice fed chow or vitamin A-deficient diet with or without β-carotene. The atherosclerotic lesion area was quantified at the aortic sinus by oil-red O staining of the lipids after 15 weeks of treatment (a). One representative aortic sinus lesion section is shown for each treatment group (magnification ×40). Red color indicates the presence of atherosclerotic lesions (b). The vitamin A-deficient diet significantly increased the atherosclerotic lesion area while β-carotene reverts this effect. Values are means ± SE, = 13–15. Within the graph, means without a common letter differ, . Dunaliella treated group (BC); vitamin A-deficient diet group (VAD); vitamin A-deficient diet fortified with Dunaliella group (VAD-BC).

(a)

(b)

(c)

(d)

Figure 5

Liver gene expression in apoE^−/− mice fed chow or vitamin A-deficient diet with or without β-carotene. Liver mRNA levels of the indicated genes were detected by real-time PCR. Gapdh was used as a reference gene. Gene expression was detected after 15 weeks of treatment. Cyp26a1 (a), Pparγ (b), Cyp7α(c), and Hmgr (d). Measured by real-time PCR and normalized to GAPDH. Values are means ± SE, = 8. Within each graph, means without a common letter differ, . Dunaliella treated group (BC); vitamin A-deficient diet group (VAD); vitamin A-deficient diet fortified with Dunaliella group (VAD-BC).

3.3. Dietary Vitamin A Deficiency Did Not Induce Insulin Resistance

As retinoids have been shown to impact glucose metabolism, we assessed whether a vitamin A-deficient diet affects glucose concentrations and insulin resistance in mice. The GTT showed that a vitamin A-deficient diet did not induce insulin resistance (data not shown).

3.4. The Effect of Vitamin A-Deficient Diet and BC Supplementation on Liver Gene Expression

In an effort to elucidate the mechanisms of cholesterol elevation and accelerated atherogenesis in VAD-treated mice, we measured the liver mRNA levels of several genes related to retinoids and cholesterol metabolism. Cyp26a1 regulates the cellular concentrations of RA via oxidation and its expression levels are used as a sensitive indicator for tissue RA concentration [28]. Its lower mRNA levels in VAD-treated mice indicate lower RA in the liver of VAD group. It is noteworthy that a diet rich in Dunaliella powder rich in β-carotene isomers prevented this decrease (Figure 5). Peroxisome proliferator-activated receptor gamma (Pparγ) participates in adipocyte differentiation, glucose and insulin homeostasis, and inflammation, and it was suggested that it plays an important role in several diseases, including obesity, diabetes, and atherosclerosis [29]. The mRNA levels of Pparγ decreased twofold in VAD-treated mice compared to the control and increased slightly (not significant) in the VAD-BC group.

Cholesterol-7-alpha-hydroxylase (Cyp7α) catalyzes the formation of 7-alpha-hydroxycholesterol, which is a rate-limiting step in the synthesis of bile acids from cholesterol. The rate-limited enzyme during cholesterol biosynthesis is 3-hydroxy-3-methyl-glutaryl-CoA reductase (Hmgr). In VAD-treated mice, Cyp7α mRNA increased approximately twofold and Hmgr decreased approximately threefold, probably as a feedback reaction to the elevated plasma cholesterol concentrations. Supplementation of BC to vitamin A-deficient diet significantly decreased Cyp7α mRNA levels possibly due to lower plasma cholesterol concentrations, while Hmgr mRNA levels were not affected.

4. Discussion

In this study, we showed that dietary vitamin A deficiency significantly accelerates atherogenesis in an atherosclerosis prone, apoE^−/− mouse model, despite unaffected plasma retinol and RA concentrations. We also found that a diet enriched with β-carotene can compensate for the effects of dietary vitamin A deficiency with regard to atherosclerosis development.

Analyzing the hepatic retinol reservoir, as an indicator for the available vitamin A in the animals, revealed a 30% hepatic total retinol pool after 15 weeks of vitamin A-deficient diet in comparison to mice fed a vitamin A-containing diet. This reduction is lower than the drop reported in other mouse strains fed with a vitamin A-deficient diet, SENCAR mice [30], aryl hydrocarbon receptor- (AHR-) null mice [31], and BALB/c mice [32]. The reason for the higher remaining pool in apoE^−/− mice is not known. As expected, decreased retinol concentrations were also detected in stellate cells isolated from the VAD group. As β-carotene is a source of vitamin A in most diets, including human diets [33], and β-carotene from Dunaliella was shown to be a precursor for retinol and retinyl esters in rats [34], we assumed that fortification of the vitamin A-deficient diet with β-carotene from Dunaliella would restore liver retinoids. Interestingly, fortification of the vitamin A-deficient diet with β-carotene isomers did not completely restore liver retinol and only a trend towards elevated total retinol concentrations was detected. Thus, liver retinol pool was comparable in the VAD and VAD-BC groups. We used Cyp26a1 to monitor liver RA concentrations [28] and as expected, VAD group had significantly lower mRNA levels of Cyp26a1, indicating lower levels of RA, and BC reverts this effect. The lack of effect of the treatments on plasma retinol or RA levels suggests dissociation between their plasma levels and liver concentrations at these diet regimes.

The two main isomers of β-carotene, all-trans and 9-cis, were accumulated in the liver as in rats fed Dunaliella bardawil powder [34] and in LDLR^−/− mice [6]. Lack of vitamin A in the diet caused significant hepatic elevation in 9-cis and all-transβ-carotene concentrations after β-carotene isomers supplementation compared to a vitamin A-containing diet enriched with these isomers. This may indicate that a lack of vitamin A in the diet and the ensuing liver reservoir decline resulted in better absorption and/or storage of β-carotene isomers. As retinoids are known to regulate the expression of carotenoid transporters [35], the higher concentrations of β-carotene isomers in the livers of the VAD group may be a result of higher expression of transporters involved in carotenoid absorption.

Plasma cholesterol concentrations were significantly increased in mice that were fed the vitamin A-deficient diet compared to the control group. These results contradict previous findings by Gatica et al., which showed no change in LDL and VLDL cholesterol concentrations in rats fed a vitamin A-deficient diet [19]. Similarly, Oliveros et al. demonstrated cholesterol and triglyceride concentration decrement in rats fed a vitamin A-deficient diet [20]. It is important to note that these experiments were conducted in animal models with a normal lipid profile, whereas we, for the first time, investigated the effects of vitamin A deficiency in a hyperlipidemic, atherosclerosis prone mouse model. The cholesterol elevation can be derived from increased synthesis, increased cholesterol absorption, or reduced plasma cholesterol clearance. In the analysis of Hmgr liver mRNA levels, we found a significant decrease in the VAD group. It seems that this decrease in liver Hmgr mRNA expression in the vitamin A-deficient diet was due to a feedback inhibition caused by the elevated plasma cholesterol concentrations in this group. A similar mechanism may have caused elevation in liver Cyp7α mRNA expression in the VAD group (the first enzyme in the cholesterol-bile acid pathway), probably in order to decrease cholesterol concentrations by turning it into bile acids. These results suggest that the increased concentrations of cholesterol are not due to increased cholesterol synthesis in the liver or decreased bile acid synthesis from cholesterol. The direct reason to the elevated plasma cholesterol in the VAD group is not known. However, the loss of retinol from HSC in the VAD group is an indication for stellate cell activation and development of liver inflammation [36]. As liver inflammation is associated with dyslipidemia [37] and has been suggested to contribute to atherosclerosis [38], we assume that it also affects cholesterol metabolism and contributed to atherogenesis in our mouse model.

The results clearly show that dietary vitamin A deficiency significantly increased the atherosclerotic lesion area, despite the lack of effects on plasma concentrations of retinol and all-trans RA. Studies on the effects of VAD on atherosclerosis have not been published and the only study investigating the effects on lipid accumulation in the blood vessels, by Gatica et al., demonstrated that the concentrations of triglycerides and total cholesterol increased in the aorta of rats treated with a vitamin A-deficient diet [39]. The results of the current study are in accordance with previous studies from our laboratory demonstrating that fortification of a diet containing vitamin A with the provitamin A carotenoids inhibits atherosclerosis in apoE^−/− and LDLR^−/− mouse models [5, 6].

It was unexpected to find that the remaining 30% liver retinoids reservoir did not prevent the increment in plasma cholesterol concentrations and accelerated atherogenesis and that dietary β-carotene can inhibit the accelerated atherogenesis without restoring the liver retinoid stockpile or increasing plasma retinoid concentrations. D'Ambrosio et al., in their review on vitamin A metabolism [40], highlighted the importance of postprandial retinyl ester uptake into extrahepatic tissues and suggested that this pathway can sufficiently provide tissue requirements. This opinion is supported by findings showing that humans who lack retinol binding protein (RBP4) [17] as well as RBP-deficient mice [18] are physiologically normal (RBP4-deficient mice). We suggest that dietary BC can supply vitamin A to peripheral tissue directly, as intact BC molecule, or by its conversion to retinyl ester in the intestine or in peripheral tissues expressing BCMO1. In a recent study we showed that BCMO1 is expressed and is active in macrophages (accepted PLOS1) and previous studies demonstrated its expression in adipocytes [41]. Hence, delivery of BC and its conversion to retinoids in BCMO1-expressing cells in peripheral tissues can potentially retard atherosclerosis without restoring liver retinol pools.

In summary, using an atherosclerotic mouse model, we demonstrated that a diet low in vitamin A can be indicated as a potential risk factor for atherosclerosis. Moreover, we suggest that dietary carotenoids as a sole source for vitamin A can compensate for vitamin A deficiency.

Abbreviations

:	Apolipoprotein E deficient
BC:	Dunaliella treated group
BCMO1:	β-carotene 15,15′ monooxygenase 1
HSC:	Hepatic stellate cell
RA:	Retinoic acid
VAD:	Vitamin A-deficient diet group
VAD-BC:	Vitamin A-deficient diet fortified with Dunaliella group.

Conflict of Interests

The authors declare that there is no conflict of interests regarding the publication of this paper.

Authors’ Contribution

Noa Zolberg Relevy, Dror Harats, Ayelet Harari, Ami Ben-Amotz, and Aviv Shaish designed the research; Noa Zolberg Relevy, Ralph Rühl, and Aviv Shaish conducted the research; Noa Zolberg Relevy and Aviv Shaish analyzed the data; Noa Zolberg Relevy, Dror Harats, Ayelet Harari, Ralph Rühl, Rafael Bitzur, Ami Ben-Amotz, and Aviv Shaish wrote the paper; Noa Zolberg Relevy, Dror Harats, and Aviv Shaish had primary responsibility for the final content. All authors read and approved the final paper.

Acknowledgments

The authors thank Dr. Esfir Ulman, Dr. Shirley Abramovitch, and Prof. Shimon Reif for technical assistance. The research was supported by Nikken Sohonsha Corporation, Gifu, Japan.

References

E.-J. Rhee, S. Nallamshetty, and J. Plutzky, “Retinoid metabolism and its effects on the vasculature,” Biochimica et Biophysica Acta, vol. 1821, no. 1, pp. 230–240, 2012.
View at: Publisher Site | Google Scholar
D. I. Axel, A. Frigge, J. Dittmann et al., “All-trans retinoic acid regulates proliferation, migration, differentiation, and extracellular matrix turnover of human arterial smooth muscle cells,” Cardiovascular Research, vol. 49, no. 4, pp. 851–862, 2001.
View at: Publisher Site | Google Scholar
B. Zhou, Y. Pan, Z. Hu et al., “All-trans-retinoic acid ameliorated high fat diet-induced atherosclerosis in rabbits by inhibiting platelet activation and inflammation,” Journal of Biomedicine and Biotechnology, vol. 2012, Article ID 259693, 9 pages, 2012.
View at: Publisher Site | Google Scholar
T. Langmann, G. Liebisch, C. Moehle et al., “Gene expression profiling identifies retinoids as potent inducers of macrophage lipid efflux,” Biochimica et Biophysica Acta: Molecular Basis of Disease, vol. 1740, no. 2, pp. 155–161, 2005.
View at: Publisher Site | Google Scholar
A. Harari, R. Abecassis, N. Relevi et al., “Prevention of atherosclerosis progression by 9-cis-β-carotene rich alga Dunaliella in apoE-deficient mice,” BioMed Research International, vol. 2013, Article ID 169517, 7 pages, 2013.
View at: Publisher Site | Google Scholar
A. Harari, D. Harats, D. Marko et al., “A 9-cis β-carotene-enriched diet inhibits atherogenesis and fatty liver formation in LDL receptor knockout mice,” The Journal of Nutrition, vol. 138, no. 10, pp. 1923–1930, 2008.
View at: Google Scholar
T. Grune, G. Lietz, A. Palou et al., “β-carotene is an important vitamin A source for humans,” The Journal of Nutrition, vol. 140, no. 12, pp. 2268S–2285S, 2010.
View at: Publisher Site | Google Scholar
S. A. Ross, P. J. McCaffery, U. C. Drager, and L. M. de Luca, “Retinoids in embryonal development,” Physiological Reviews, vol. 80, no. 3, pp. 1021–1054, 2000.
View at: Google Scholar
J. H. Humphrey, K. P. West Jr., and A. Sommer, “Vitamin A deficiency and attributable mortality among under-5-year-olds,” Bulletin of the World Health Organization, vol. 70, pp. 225–232, 1992.
View at: Google Scholar
A. Bousvaros, D. Zurakowski, C. Duggan et al., “Vitamins A and E serum levels in children and young adults with inflammatory bowel disease: effect of disease activity,” Journal of Pediatric Gastroenterology and Nutrition, vol. 26, no. 2, pp. 129–135, 1998.
View at: Publisher Site | Google Scholar
M. A. Leo and C. S. Lieber, “Hepatic vitamin A depletion in alcoholic liver injury,” The New England Journal of Medicine, vol. 307, no. 10, pp. 597–601, 1982.
View at: Publisher Site | Google Scholar
A. Lindqvist, J. Sharvill, D. E. Sharvill, and S. Andersson, “Loss-of-function mutation in carotenoid 15,15′-monooxygenase identified in a patient with hypercarotenemia and hypovitaminosis A,” The Journal of Nutrition, vol. 137, no. 11, pp. 2346–2350, 2007.
View at: Google Scholar
M. C. Sales, A. de Azevedo Paiva, D. de Queiroz, R. A. F. Costa, M. A. L. da Cunha, and D. F. Pedraza, “Nutritional status of iron in children from 6 to 59 months of age and its relation to vitamin A deficiency,” Nutricion Hospitalaria, vol. 28, no. 3, pp. 734–740, 2013.
View at: Publisher Site | Google Scholar
D. W. Goodman, H. S. Huang, and T. Shiratori, “Tissue distribution and metabolism of newly absorbed vitamin a in the rat,” The Journal of Lipid Research, vol. 6, pp. 390–396, 1965.
View at: Google Scholar
M. Kanai, A. Raz, and D. S. Goodman, “Retinol-binding protein: the transport protein for vitamin A in human plasma,” The Journal of Clinical Investigation, vol. 47, no. 9, pp. 2025–2044, 1968.
View at: Publisher Site | Google Scholar
S. B. Kurlandsky, M. V. Gamble, R. Ramakrishnan, and W. S. Blaner, “Plasma delivery of retinoic acid to tissues in the rat,” The Journal of Biological Chemistry, vol. 270, no. 30, pp. 17850–17857, 1995.
View at: Publisher Site | Google Scholar
H. K. Biesalski, J. Frank, S. C. Beck et al., “Biochemical but not clinical, vitamin A deficiency results from mutations in the gene for retinol binding protein,” The American Journal of Clinical Nutrition, vol. 69, no. 5, pp. 931–936, 1999.
View at: Google Scholar
L. Quadro, W. S. Blaner, D. J. Salchow et al., “Impaired retinal function and vitamin A availability in mice lacking retinol-binding protein,” The EMBO Journal, vol. 18, no. 17, pp. 4633–4644, 1999.
View at: Publisher Site | Google Scholar
L. V. Gatica, V. A. Vega, F. Zirulnik, L. B. Oliveros, and M. S. Gimenez, “Alterations in the lipid metabolism of rat aorta: effects of vitamin A deficiency,” Journal of Vascular Research, vol. 43, no. 6, pp. 602–610, 2006.
View at: Publisher Site | Google Scholar
L. B. Oliveros, M. A. Domeniconi, V. A. Vega, L. V. Gatica, A. M. Brigada, and M. S. Gimenez, “Vitamin A deficiency modifies lipid metabolism in rat liver,” British Journal of Nutrition, vol. 97, no. 2, pp. 263–272, 2007.
View at: Publisher Site | Google Scholar
S. Hessel, A. Eichinger, A. Isken et al., “CMO1 deficiency abolishes vitamin a production from β-carotene and alters lipid metabolism in mice,” The Journal of Biological Chemistry, vol. 282, no. 46, pp. 33553–33561, 2007.
View at: Publisher Site | Google Scholar
A. Ben-Amotz, A. Lers, and M. Avron, “Stereoisomers of beta-carotene and phytoene in the alga Dunaliella bardawil,” Plant Physiology, vol. 86, no. 4, pp. 1286–1291, 1988.
View at: Google Scholar
A. Shaish, A. Daugherty, F. O'Sullivan, G. Schonfeld, and J. W. Heinecke, “Beta-carotene inhibits atherosclerosis in hypercholesterolemic rabbits,” The Journal of Clinical Investigation, vol. 96, no. 4, pp. 2075–2082, 1995.
View at: Publisher Site | Google Scholar
R. Rühl, “Method to determine 4-oxo-retinoic acids, retinoic acids and retinol in serum and cell extracts by liquid chromatography/diode-array detection atmospheric pressure chemical ionisation tandem mass spectrometry,” Rapid Communications in Mass Spectrometry, vol. 20, no. 16, pp. 2497–2504, 2006.
View at: Publisher Site | Google Scholar
S. L. Friedman and F. J. Roll, “Isolation and culture of hepatic lipocytes, Kupffer cells, and sinusoidal endothelial cells by density gradient centrifugation with Stractan,” Analytical Biochemistry, vol. 161, no. 1, pp. 207–218, 1987.
View at: Publisher Site | Google Scholar
A. Shaish, J. George, B. Gilburd, P. Keren, H. Levkovitz, and D. Harats, “Dietary β-carotene and α-tocopherol combination does not inhibit atherogenesis in an ApoE-deficient mouse model,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 19, no. 6, pp. 1470–1475, 1999.
View at: Publisher Site | Google Scholar
S. Ishibashi, J. Herz, N. Maeda, J. L. Goldstein, and M. S. Brown, “The two-receptor model of lipoprotein clearance: tests of the hypothesis in “knockout” mice lacking the low density lipoprotein receptor, apolipoprotein E, or both proteins,” Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 10, pp. 4431–4435, 1994.
View at: Publisher Site | Google Scholar
Y. Yamamoto, R. Zolfaghari, and A. C. Ross, “Regulation of CYP26 (cytochrome P450RAI) mRNA expression and retinoic acid metabolism by retinoids and dietary vitamin A in liver of mice and rats,” The FASEB Journal, vol. 14, no. 13, pp. 2119–2127, 2000.
View at: Publisher Site | Google Scholar
R. Stienstra, C. Duval, M. Müller, and S. Kersten, “PPARs, obesity, and inflammation,” PPAR Research, vol. 2007, Article ID 95974, 10 pages, 2007.
View at: Publisher Site | Google Scholar
T. Kuwata, I.-M. Wang, T. Tamura et al., “Vitamin A deficiency in mice causes a systemic expansion of myeloid cells,” Blood, vol. 95, no. 11, pp. 3349–3356, 2000.
View at: Google Scholar
F. Andreola, D. F. Calvisi, G. Elizondo et al., “Reversal of liver fibrosis in aryl hydrocarbon receptor null mice by dietary vitamin A depletion,” Hepatology, vol. 39, no. 1, pp. 157–166, 2004.
View at: Publisher Site | Google Scholar
C. B. Stephensen, S. R. Blount, T. R. Schoeb, and J. Y. Park, “Vitamin A deficiency impairs some aspects of the host response to influenza A virus infection in BALB/c mice,” Journal of Nutrition, vol. 123, no. 5, pp. 823–833, 1993.
View at: Google Scholar
J. C. Bauernfeind, “Carotenoids as colorants and vitamin A precursors,” in Technological and Nutritional Applications, H. A. Klaui, C. R. Adams, and W. L. Marusich, Eds., 1981.
View at: Google Scholar
A. Ben-Amotz, S. Mokady, and M. Avron, “The β-carotene-rich alga Dunaliella bardawil as a source of retinol in a rat diet,” British Journal of Nutrition, vol. 59, no. 3, pp. 443–449, 1988.
View at: Publisher Site | Google Scholar
E. Reboul, “Absorption of vitamin A and carotenoids by the enterocyte: focus on transport proteins,” Nutrients, vol. 5, no. 9, pp. 3563–3581, 2013.
View at: Publisher Site | Google Scholar
R. Blomhoff and K. Wake, “Perisinusoidal stellate cells of the liver: important roles in retinol metabolism and fibrosis,” The FASEB Journal, vol. 5, no. 3, pp. 271–277, 1991.
View at: Google Scholar
S. Fargion, M. Porzio, and A. L. Fracanzani, “Nonalcoholic fatty liver disease and vascular disease: state-of-the-art,” World Journal of Gastroenterology, vol. 20, pp. 13306–13324, 2014.
View at: Google Scholar
R. Kleemann, L. Verschuren, M. J. Van Erk et al., “Atherosclerosis and liver inflammation induced by increased dietary cholesterol intake: a combined transcriptomics and metabolomics analysis,” Genome Biology, vol. 8, article R200, 2007.
View at: Publisher Site | Google Scholar
L. Gatica, S. Alvarez, N. Gomez et al., “Vitamin A deficiency induces prooxidant environment and inflammation in rat aorta,” Free Radical Research, vol. 39, no. 6, pp. 621–628, 2005.
View at: Publisher Site | Google Scholar
D. N. D'Ambrosio, R. D. Clugston, and W. S. Blaner, “Vitamin A metabolism: an update,” Nutrients, vol. 3, no. 1, pp. 63–103, 2011.
View at: Publisher Site | Google Scholar
G. P. Lobo, J. Amengual, H. N. Li et al., “β,β-carotene decreases peroxisome proliferator receptor γ activity and reduces lipid storage capacity of adipocytes in a β,β-carotene oxygenase 1-dependent manner,” The Journal of Biological Chemistry, vol. 285, no. 36, pp. 27891–27899, 2010.
View at: Publisher Site | Google Scholar

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Copyright © 2015 Noa Zolberg Relevy et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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BioMed Research International

Vitamin A-Deficient Diet Accelerated Atherogenesis in Apolipoprotein E−/− Mice and Dietary β-Carotene Prevents This Consequence

Abstract

1. Introduction

2. Materials and Methods

2.1. Mice

2.2. Diet

2.3. Study Design

2.4. Lipid Analysis

2.5. Carotenoid Analysis

2.6. Retinol Analysis

2.7. Retinoic Acid Analysis

2.8. Isolation of Primary Mouse Hepatic Stellate Cells

2.9. Glucose Tolerance Test

2.10. Assessment of Atherosclerosis in the Aortic Sinus

2.11. Fast Protein Liquid Chromatography Analysis of Lipoproteins

2.12. Analysis of Gene Expression by Real-Time PCR

2.13. Statistical Analyses

3. Results

3.1. Vitamin A-Deficient Diet Reduced Liver Retinol Content

3.2. Vitamin A-Deficient Diet Increased Plasma Cholesterol Concentrations and Accelerated Atherosclerosis While Dietary β-Carotene Reversed These Effects

3.3. Dietary Vitamin A Deficiency Did Not Induce Insulin Resistance

3.4. The Effect of Vitamin A-Deficient Diet and BC Supplementation on Liver Gene Expression

4. Discussion

Abbreviations

Conflict of Interests

Authors’ Contribution

Acknowledgments

References

Copyright

Vitamin A-Deficient Diet Accelerated Atherogenesis in Apolipoprotein E^−/− Mice and Dietary β-Carotene Prevents This Consequence