Protection against Amoebic Liver Abscess in Hamster by Intramuscular Immunization with an Autographa californica Baculovirus Driving the Expression of the Gal-Lectin LC3 Fragment

Figure 3

Cellular anti-E. histolytica responses in AcNPV-LC3 intramuscular immunized and challenged hamsters. Cellular immune response was evaluated in splenocytes after immunization and challenge (day 49) by staining of the harvested cells with CFSE and stimulation with E. histolytica total extract during 72 h followed by flow cytometry analysis. In (a), the first panel shows dot plot of splenocytes stained with CFSE without antigenic stimulation. The cell population selected according to their size and granularity (P1 region) and its corresponding dot plot and histogram are shown (a). (b) shows a representative dot plot and histogram of IM AcNPC-LC3 splenocytes from nonprotected (upper) and sterile protected (bottom) hamsters. (c) shows the proliferation rate of all experimental groups classified by protection level. Bars represent mean of percentage of proliferation (; ).

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