BioMed Research International / 2015 / Article / Fig 1

Research Article

Regulation of Cell Cycle Regulators by SIRT1 Contributes to Resveratrol-Mediated Prevention of Pulmonary Arterial Hypertension

Figure 1

PDGF-BB affects cell cycle regulatory molecules and SIRT1 expressions. After 24-hour serum starvation, HPASMCs were treated with 10 ng·mL−1 PDGF-BB and harvested at the indicated time points. p21, cyclin D1, cyclin E (a), and SIRT1 (c) protein levels were analyzed by western blotting. Bar graphs show densitometric analysis of western blotting. The densitometric quantification was normalized to GAPDH. Data are shown as means ± SEM for three independent experiments. SIRT1 mRNA level was analyzed by real-time PCR (b) . mRNA level was normalized to the internal control -actin. , , versus 0 hour.

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