Characterization of Bone Marrow Mononuclear Cells on Biomaterials for Bone Tissue Engineering In Vitro

Figure 1

Seeding efficacy of BMC depending on the surface coating and medium conditions. BMCs () were seeded on β-TCP either coated with fibronectin or human fresh frozen plasma and on uncoated β-TCP in the presence of MesenCult medium or DMEM. Seeding efficacy is plotted on the ordinate. No significant differences in seeding efficacy were observed (a). Representative fluorescence microscopy images of DAPI stained BMC seeded under the different conditions are shown in (b). Similar amounts of adhering BMC were seen under each condition. No selective enrichment or depletion of specific progenitor cell subpopulation was observed on the surface of all biomaterials in various medium conditions as shown in (c). The percentages of progenitor cell types which resemble EPC (CD34+/CD45+; CD34+/CD133+/CD45+) and MSC (CD45−/CD271+) were analyzed in the fraction of nonadhering cells. The similar values indicate that no selective enrichment of progenitor cell subpopulations occurred due to the different culture and surface conditions.

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