Characterization of Bone Marrow Mononuclear Cells on Biomaterials for Bone Tissue Engineering In Vitro

Figure 4

Metabolic activity and VEGF release of BMC depending on biomaterials over a 21-day observation period. (a) Metabolic activity was measured by MTT-assay as described in Materials and Methods (). DBM day 14 versus bovine cancellous bone (BS); DBM day 21 versus β-TCP and bovine cancellous bone. The release of VEGF to the medium is shown in (b). VEGF in the supernatant was assessed by ELISA as described in Materials and Methods section. The VEGF secretion dropped significantly from day 2 to day 21. A trend to higher values on day 2 was seen in BMC cultured on DBM in comparison to BMC seeded on bovine cancellous bone (, ).

(a)

(b)