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Primers, PCR products, or bacteriocins | Nucleotide sequence (5′-3′) or description | Amplifications |
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Primers | | |
S602-F | GCCATGAGCTCGAATTCTCGAGAAAAG | R-SRCAM602, R-E760, R-L1077 |
S071-F | GTCCAGAGCTCGAATTCTCGAGAAAAG | R-SRCAM 602, R-E760, R-L1077, R-OR7 |
SARP-R | AGGTACCATAAGTTGCGGCCGC | R-OR7 |
ALFA-F | TACTATTGCCAGCATTGCTGC | pPICZαA amplification fragment including the cloned synthetic gene |
3AOX1-R | GCAAATGGCATTCTGACATCC | pPICZαA amplification fragment including the cloned synthetic gene |
PCR products | | |
R-SRCAM602 | 136-bp XhoI/NotI fragment containing the α-factor Kex2 signal cleavage fused to the mature synthetic srcam602 gene with the P. pastoris codon usage | |
R-OR7 | 181-bp XhoI/NotI fragment containing the α-factor Kex2 signal cleavage fused to the mature synthetic or-7 gene with the P. pastoris codon usage | |
R-E760 | 242-bp XhoI/NotI fragment containing the α-factor Kex2 signal cleavage fused to the mature synthetic e-760 gene with the P. pastoris codon usage | |
R-L1077 | 167-bp XhoI/NotI fragment containing the α-factor Kex2 signal cleavage fused to the mature synthetic l-1077 gene with the P. pastoris codon usage | |
Bacteriocins | | |
BacSRCAM602 (amino acid sequence) | ATYYGNGLYCNKQKHYTWVDWNKASR EIGKITVNGWVQH | |
BacSRCAM602 (P. pastoris codon usage) | gctacttactacggtaacggtctttactgtaacaagcagaagcact acacttgggttgactggaacaaggcttccagagagatcggtaag atcactgttaacggttgggttcaaca | |
BacOR-7 (amino acid sequence) | KTYYGTNGVHCTKNSLWGKVRLKNMK YDQNTTYMGRLQDILLGWATGAFGKTFH |
BacOR-7 (P. pastoris codon usage) | aagacttactacggaactaacggtgttcactgtactaagaattcctt gtggggtaaggttagattgaagaacatgaagtacgaccagaaca ctacttacatgggtagattgcaggacatcttgttgggttgggctact ggtgctttcggtaagacttttcat |
BacE-760 (amino acid sequence) | NRWYCNSAAGGVGGAAVCGLAGYVGE AKENIAGEVRKGWGMAGGFTHNKACKS FPGSGWASG |
BacE-760 (P. pastoris codon usage) | aacagatggtactgtaactccgctgctggtggtgttggtggtgct gctgtttgtggtttggctggttatgttggtgaggctaaagaaaacat tgctggtgaggttagaaagggttggggtatggctggtggtttcac tcataacaaggcttgtaagtccttcccaggttctggttgggcttctggt |
BacL-1077 (amino acid sequence) | TNYGNGVGVPDAIMAGIIKLIFIFNIRQGY NFGKKAT |
BacL-1077 (P. pastoris codon usage) | actaactacggtaacggtgttggtgttccagacgctattatggctg gtatcatcaagttgatcttcatcttcaacatcagacagggttacaac ttcggtaagaaggctact |
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