Cloning and Expression of Synthetic Genes Encoding the Broad Antimicrobial Spectrum Bacteriocins SRCAM 602, OR-7, E-760, and L-1077, by Recombinant Pichia pastoris

Table 4

Antimicrobial activity of fractions generated during purification of supernatants from P. pastoris X-SRCAM602, P. pastoris X-33OR-7, P. pastoris X-33E-760, and P. pastoris X-33L-1077, grown in BMMY with methanol.

Strain	Antimicrobial activity (BU/mL) of the purified fractions^a
	SN	AS	GF	SE	OE	RP-FPLC
P. pastoris X-33SRCAM602	NA	NA	NA	NA	12,800	1,106,531
P. pastoris X-33OR-7	NA	NA	127	32	1,391	10,027
P. pastoris X-33E-760	NA	NA	NA	NA	687	14,442
P. pastoris X-33L-1077	NA	NA	35	23	2,069	13,213

Most of the data are mean from two independent determinations in triplicate.
^aAntimicrobial activity against Pediococcus damnosus CECT4797 as determined by microtiter plate assay (MPA). BU: bacteriocin units. NA: no activity.
Purification fractions: SN: supernatant; AS: ammonium sulfate precipitation; GF: gel filtration; SE: Sepharose fast flow eluate; OE: Octyl Sepharose eluate; RP-FPLC: reversed-phase eluate.