Heterologous Reconstitution of Omega-3 Polyunsaturated Fatty Acids in Arabidopsis

Table 2

Primers used for this study. The data include sequences and annealing temperatures () for the primer pairs.

Primer set	Primer name	Sequence ()
I	McD6D-106F	TGG TTG GTA ATC GAC AGA AAG GTG T	65°C
	McD6D-730R	TCT TTC CTA GCA CAA AGG TGT GGA G
	AsELO-ORF-F	ATGGAGACCTTCAATCACAAACTGAACGTTTAC
	AsELO-ORF-R	TCAATCCACTCTCAGTTTCTTGTGTGCAGTGT
	PtD5D-ORF-F	ATGGCTCCGGATGCGGATAAGCTT
	PtD5D-ORF-R	TTACGCCCGTCCGGTCAAGGGATTTT
II	Hind-McD6D-F	CCC AAG CTT ATG GGA GGT GGA GGC CAG	62°C
	BamH-McD6D-R	CGG GAT CCT CAT TTA TGG AGA TAA GCA TCC
	Hind-AsELO-F	CCC AAG CTT ATG GAG ACC TTC AAT CAC
	BamH-AsELO-R	CGG GAT CCT CAA TCC ACT CTC AGT TT
	Cla-PtD5D-F	CTT TAT TCA TCG ATA TGG CTC CGG ATG CGG AT
	BamH-PtD5D-R	GCA GGA CTC TAG GAT CCT TAC GCC CGT CCG GTC AA
III	Infu-AsELOVL5-F	GAT TAC GAA TTC GAG CTC GCG GCC GCT AAT ATT TTG CAA AAA	68°C
	Infu-AsELOVL5-R	CCC CGG GTA CCG AGC TCG TCC TGC TGA GCC TCG A
	Infu-PtD5D-F	GCG AAG AGG CCC GCA CTA CGT ATA ATA TTT TGC AAA AAG A
	Infu-PtD5D-R	CAA CTG TTG GGA AGG GCC AAT TGG TCC TGC TGA GCC TCG A
qPCR	AtACT1 537F	TCT TGA TCT TGC TGG TCG TG	60°C
	AtACT1 707R	GAG CTG GTT TTG GCT GTC TC
	McD6D 223F	TTC CAT CCA GAG CCT GAC TT
	McD6D 371R	CCC TCC CTC TCT ACC TCC TC
	AsELO 720F	CAC GCT AAT TTT CCT GTT CTC A
	AsELO 871R	GTT TCT TGT GTG CAG TGT GCT
	PtD5D 782F	GAT ACT GGT TGT CCG CTG TCT
	PtD5D 935R	ATC ACG TTC ACC GCA ATG TA

Restriction enzyme sites are underlined.