Effect of Metformin on Viability, Morphology, and Ultrastructure of Mouse Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells and Balb/3T3 Embryonic Fibroblast Cell Line
Characterization of BMSC phenotype and determination their multipotency. Phenotype of BMSCs was determined by flow cytometry. The analysis revealed that BMSCs isolated by the described method were negative for CD45 and CD31. In addition, the cells were strongly positive for CD51, CD73, CD90, and CD105 (panel (a)). Morphology of BMSC culture in standard (b), adipogenic (c), and osteogenic conditions (d). Specific stainings were carried out to determine the BMSC differentiation to adipocytes and osteocytes. Oil-Red O staining was used to detect lipid droplet formation during adipogenic differentiation (c). Alizarin red staining was used to detect calcium deposition during osteogenic differentiation (d). Images of differentiated cultures were captured at 100x magnification (scale bar = 200 μm).