Effect of Metformin on Viability, Morphology, and Ultrastructure of Mouse Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells and Balb/3T3 Embryonic Fibroblast Cell Line

Figure 2

Influence of metformin on the proliferative activity of BMSCs (a) and mouse embryonic fibroblast cell line Balb/3T3 (b). Proliferation of control cultures was compared to the cultures propagated with metformin. (a) No difference in the proliferation rate of BMSCs was recorded after 24 and 48 hours () when cells were cultured with the addition of 1 mM and 5 mM metformin. A significant decrease in the proliferative activity of BMSCs treated with 1 mM and 5 mM concentrations was noticed after 72 hours. The addition of 10 mM metformin to the BMSC culture significantly reduced the proliferation of BMSCs from the second day of culture. (b) Proliferative activity of Balb/3T3 in experimental cultures decreased significantly after 48 hours. Statistically significant differences in the experimental and control culture of Balb/3T3 were also observed after 72 hours of propagation. An asterisk (*) indicates a statistically significant difference (). Significance was determined by two-way ANOVA test. Each test included a blank containing complete medium without cells. The -axis refers to the time of cell propagation, while mark on -axis refers to the difference between absorbance read at 600 nm and 690 nm, including blank sample.

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