Effect of Metformin on Viability, Morphology, and Ultrastructure of Mouse Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells and Balb/3T3 Embryonic Fibroblast Cell Line

Figure 5

The percentage of dead cells quantified after propidium iodide staining. Calculation was performed based on the images obtained from three independent experiments. Evaluation of cell viability in cultures after 48 h of treatment with 1 mM, 5 mM, and 10 mM metformin. The number of dead cells in BMSC cultures (a) and Balb/3T3 cultures (b) increased after treatment with metformin at 5 and 10 mM concentration. Statistical analysis was performed in relation to the results obtained for control culture (no metformin). An asterisk (*) indicates a statistically significant difference (). Significance was determined by one-way ANOVA test.

(a)

(b)