SimFuse: A Novel Fusion Simulator for RNA Sequencing (RNA-Seq) Data

Figure 1

Definition of essential terms. (a) Definition of fragment, read, and insert size. A fragment is sequenced in both directions to get a pair of reads in the paired-end sequencing. The size of the nonsequenced portion between the paired ends is called insert size. (b) Definition of spanning read and splitting read pairs. The fusion boundaries are in the insert region in a spanning read pair, while the fusion boundaries fall within one of the reads in a splitting read pair.