Paricalcitol Inhibits Aldosterone-Induced Proinflammatory Factors by Modulating Epidermal Growth Factor Receptor Pathway in Cultured Tubular Epithelial Cells

Figure 2

ADAM17 inhibition blocks aldosterone-mediated EGFR activation in tubular epithelial cells. HK2 cells were transfected with an ADAM17 siRNA or scrambled siRNA, as described in “methods,” before stimulation with 1 μmol/L Aldo for 15 min. Phosphorylated-EGFR levels were evaluated by Western blot using an antibody against Y1068 phosphorylated-EGFR (p-EGFR1068). GAPDH levels were used as loading control and ADAM17 as silencing control. (a) Representative Western blot and quantification of data expressed as mean ± SEM of 4 independent experiments. versus untreated siRNA control-transfected cells control. ^# versus Aldo-treated siRNA scramble cells. (b) Cells were preincubated with the specific ADAM17 inhibitor TAPI-2 (50 μmol/L) for 1 hour and then stimulated with 1 μmol/L Aldo for 15 min. Representative Western blot and data expressed as mean ± SEM of 4 independent experiments. versus control. ^# versus Aldo.

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