Paricalcitol Inhibits Aldosterone-Induced Proinflammatory Factors by Modulating Epidermal Growth Factor Receptor Pathway in Cultured Tubular Epithelial Cells

Figure 6

Aldosterone activates ERK and STAT pathways via ADAM-17/TGF-α/EGFR axis. HK2 cells were preincubated with (a) the specific ADAM17 inhibitor TAPI-2 (50 μmol/L; 1 hour) or (b) a neutralizing antibody against TGFα (2.5 μg/mL, 24 hour) and then stimulated with 1 μmol/L Aldo for 15 min. Figures (a) and (b) show representative Western blot experiment and (c) data expressed as mean ± SEM of 3 experiments. versus control. ^# versus Aldo.

(a)

(b)

(c)