Paricalcitol Inhibits Aldosterone-Induced Proinflammatory Factors by Modulating Epidermal Growth Factor Receptor Pathway in Cultured Tubular Epithelial Cells

Figure 8

Paricalcitol inhibits aldosterone-induced activation of ADAM-17/TGF-α/EGFR signalling pathway. Cells were pretreated with the analogue of vitamin D Paricalcitol (12 μmol/L) for 48 hours before stimulation with 1 μmol/L Aldo for 15 min (protein) or 6 hours (gene studies). Paricalcitol inhibits Aldo-induced EGFR activation (a, b) and gene overexpression of TGFα and HB-EGF (c). Figure (a) shows a representative Western blot, in (b) quantification of p-EGFR and in (c) gene expression levels by real-time PCR. Paricalcitol restores Aldo-induced changes in VDR protein (a, d) and gene (e) expression levels. Figure (a) shows a representative Western blot, in (d) quantification of VDR protein levels and in (e) gene expression levels. Data are expressed as mean ± SEM of 3 independent experiments. versus control. ^# versus Aldo.

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