Influence of Culture Media on Biofilm Formation by Candida Species and Response of Sessile Cells to Antifungals and Oxidative Stress

Figure 5

Susceptibility of sessile and planktonic cells to azole-derived antifungals and AgNPs. Planktonic (either from logarithmic or stationary phase) and sessile cells of C. albicans were diluted to an 0.1 with sterile deionized water. (a) Aliquots of dilutions were incubated at 37°C in the presence of increasing concentrations of the indicated antifungals or (b) antifungals plus AgNPs. After 90 min, cells were pelleted by low-speed centrifugation and resuspended in sterile deionized water to an 0.1. LP, logarithmic phase; SP, stationary phase; B, biofilm; AgNPs, silver nanoparticles; S, Sabouraud medium; MH, Mueller-Hinton medium.

(a)

(b)