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BioMed Research International
Volume 2015, Article ID 785474, 9 pages
http://dx.doi.org/10.1155/2015/785474
Research Article

Optimizing Perfusion-Decellularization Methods of Porcine Livers for Clinical-Scale Whole-Organ Bioengineering

1Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China
2Department of Pathology and Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China
3Laboratory of Pathology and Department of Liver Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China

Received 7 September 2014; Revised 3 March 2015; Accepted 3 March 2015

Academic Editor: Antonio Salgado

Copyright © 2015 Qiong Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aim. To refine the decellularization protocol of whole porcine liver, which holds great promise for liver tissue engineering. Methods. Three decellularization methods for porcine livers (1% sodium dodecyl sulfate (SDS), 1% Triton X-100 + 1% sodium dodecyl sulfate, and 1% sodium deoxycholate + 1% sodium dodecyl sulfate) were studied. The obtained liver scaffolds were processed for histology, residual cellular content analysis, and extracellular matrix (ECM) components evaluation to investigate decellularization efficiency and ECM preservation. Rat primary hepatocytes were seeded into three kinds of scaffold to detect the biocompatibility. Results. The whole liver decellularization was successfully achieved following all three kinds of treatment. SDS combined with Triton had a high efficacy of cellular removal and caused minimal disruption of essential ECM components; it was also the most biocompatible procedure for primary hepatocytes. Conclusion. We have refined a novel, standardized, time-efficient, and reproducible protocol for the decellularization of whole liver which can be further adapted to liver tissue engineering.