Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2015, Article ID 785474, 9 pages
http://dx.doi.org/10.1155/2015/785474
Research Article

Optimizing Perfusion-Decellularization Methods of Porcine Livers for Clinical-Scale Whole-Organ Bioengineering

1Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China
2Department of Pathology and Laboratory of Pathology, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China
3Laboratory of Pathology and Department of Liver Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, China

Received 7 September 2014; Revised 3 March 2015; Accepted 3 March 2015

Academic Editor: Antonio Salgado

Copyright © 2015 Qiong Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1. Representative photographs of the devices for a human-size liver graft (left) and the liver perfusion culture system in an incubator (right).

Figure S2. Decellularized whole liver scaffold from a 30kg pig following a standardized human-scale whole-liver decellularization protocol. The macrographs of liver (A) before and (B) after decellularization. Scale bars: 10 cm. (C) H&E staining of decellularized liver . Scale bar: 100 μm.

  1. Supplementary Material