Metabolic Engineering of Escherichia coli for Poly(3-hydroxybutyrate) Production under Microaerobic Condition

Table 1

Strains, plasmids, and primers used in this study.

	Description	Reference
E. coli strains
BW25113	lacIq rrnBT14  ΔlacZWJ16  hsdR514  ΔaraBADAH33  ΔrhaBADLD78	[11]
BWa	BW25113 ΔackA-pta	[11]
BWap	BW25113 ΔackA-pta, ΔpoxB	[11]
BWapl	BW25113 ΔackA-pta, ΔpoxB, ΔldhA	[11]
BWapld	BW25113 ΔackA-pta, ΔpoxB, ΔldhA, ΔadhE	[11]
BWapldf	BW25113 ΔackA-pta, ΔpoxB, ΔldhA, ΔadhE, ΔpflB	[11]
Plasmids
pWYC09	pBluescript II SK^− derivatives containing phaCAB from R. eutropha with promoter PadhE, Amp^R	[10]
pBBR1MCS-2	Broad-host-range plasmid, Kan^R	[10]
pMCS2pdc	Promoter Ppdc inserted into pBBR1MCS-2	This study
pMCS2pflB	pflB inserted into pMCS2pdc	This study
Primers
pdcF	5′-ATACTCGAGTTACGCTCATGATCGCGGCATGTC
pdcR	5′-CCCCATATGTTACTCCATATATTCAAAAC
plfF	5′-GCTAGGCATATGTCCGAGCTTAATGAAAA
plfR	5′-CCGAATTCTTACATAGATTGAGTGAAGGT

All oligonucleotides were synthesized by AuGCT Biotechnology (Beijing, China). Restriction endonuclease digestion sites were underlined.