Metabolic Engineering of Escherichia coli for Poly(3-hydroxybutyrate) Production under Microaerobic Condition
Table 2
P3HB accumulation and by-product formation of E. coli BW25113 and its five mutants harboring PadhE controlled phaCAB operon.
E. coli
CDW (g/L)
P3HB content (%)
Glucose consumed (g/L)
Succinate (g/L)
Lactate (g/L)
Formate (g/L)
Acetate (g/L)
Ethanol (g/L)
BW25113
1.24 ± 0.08
44.85 ± 1.46
7.39 ± 0.09
0.34 ± 0.04
1.21 ± 0.12
0.06 ± 0.01
1.59 ± 0.08
0.25 ± 0.04
BWa
0.73 ± 0.10
68.31 ± 4.86
5.31 ± 0.16
0.37 ± 0.06
1.47 ± 0.26
0.04 ± 0.01
0.35 ± 0.02
0.19 ± 0.02
BWap
0.57 ± 0.06
70.69 ± 5.35
4.89 ± 0.24
0.28 ± 0.06
1.41 ± 0.16
0.03 ± 0.01
0.26 ± 0.06
0.21 ± 0.04
BWapl
1.33 ± 0.08
73.58 ± 4.72
6.26 ± 0.18
0.58 ± 0.08
ND
0.06 ± 0.02
0.45 ± 0.04
0.53 ± 0.06
BWapld
0.85 ± 0.04
78.84 ± 3.27
5.14 ± 0.23
0.32 ± 0.04
ND
ND
0.31 ± 0.02
ND
BWapldf
0.36 ± 0.03
24.44 ± 3.95
2.23 ± 0.08
0.18 ± 0.02
ND
ND
0.20 ± 0.05
ND
The recombinants harboring pWYC09 were cultivated in 250 mL sealed tubes completely filled with LB medium supplemented with 10 g/L glucose at 37°C for 48 h. CDW: cell dry weight. ND: not detected. Data shown are the average and standard deviation of three parallel experiments.
