Metabolic Engineering of Escherichia coli for Poly(3-hydroxybutyrate) Production under Microaerobic Condition
Table 3
Influence of pflB overexpression on cell growth, P3HB accumulation, and by-product formation.
E. coli
CDW (g/L)
P3HB content (%)
Glucose consumed (g/L)
Succinate (g/L)
Lactate (g/L)
Formate (g/L)
Acetate (g/L)
Ethanol (g/L)
BW25113
0.85 ± 0.03
38.44 ± 5.91
6.95 ± 0.17
0.68 ± 0.03
0.97 ± 0.09
0.23 ± 0.02
1.68 ± 0.01
0.45 ± 0.06
BWa
0.58 ± 0.08
71.58 ± 6.93
5.31 ± 0.13
0.72 ± 0.06
1.29 ± 0.12
0.20 ± 0.01
0.64 ± 0.06
0.29 ± 0.04
BWap
0.64 ± 0.02
66.22 ± 4.67
4.89 ± 0.12
0.64 ± 0.01
1.16 ± 0.08
0.18 ± 0.02
0.24 ± 0.07
0.25 ± 0.03
BWapl
1.12 ± 0.07
76.47 ± 2.98
5.36 ± 0.26
0.84 ± 0.02
ND
0.11 ± 0.04
0.29 ± 0.04
0.38 ± 0.05
BWapld
1.16 ± 0.05
84.79 ± 2.37
6.24 ± 0.32
0.85 ± 0.05
ND
0.25 ± 0.06
0.35 ± 0.04
ND
BWapldf
0.70 ± 0.06
44.57 ± 4.02
3.78 ± 0.26
0.73 ± 0.02
ND
0.15 ± 0.04
0.16 ± 0.05
ND
The recombinants harboring pWYC09 and pMCS2pflB were cultivated in 250 mL sealed tubes completely filled with LB medium supplemented with 10 g/L glucose at 37°C for 48 h. CDW: cell dry weight. ND: not detected. Data shown are the average and standard deviation of three parallel experiments.
